CAJ | 학술논문

目的对马尾松花粉多糖(PPM)进行硫酸酯化,得硫酸酯化多糖(S-PPM),比较酯化前后抗肿瘤活性、免疫调节活性和清除自由基能力的变化.方法 PPM采用氯磺酸-吡啶法进行硫酸酯化.结果 S-PPM取代度为1.47,S-PPM对体内外肿瘤细胞的抑制作用、对促进T、B细胞增殖作用均高于PPM;对促进巨噬细胞的吞噬作用变化不大;S-PPM清除超氧阴离子自由基的作用明显强于PPM,清除羟自由基的作用小于PPM.结论 S-PPM能促进特异性免疫抑制肿瘤细胞的增殖;硫酸酯化也能改善对多糖清除自由基的作用.
목적 대마미송화분다당(PPM)진행류산지화,득류산지화다당(S-PPM),비교지화전후항종류활성、면역조절활성화청제자유기능력적변화.방법 PPM채용록광산-필정법진행류산지화.결과 S-PPM취대도위1.47,S-PPM대체내외종류세포적억제작용、대촉진T、B세포증식작용균고우PPM;대촉진거서세포적탄서작용변화불대;S-PPM청제초양음리자자유기적작용명현강우PPM,청제간자유기적작용소우PPM.결론 S-PPM능촉진특이성면역억제종류세포적증식;류산지화야능개선대다당청제자유기적작용.
Purpose To study the polysaccharide from Pinus massoniana pollen(PPM)and to compare its anti-tumor,immune modulation activities and scavenging qualities of free radical with its sulfated derivative(S-PPM).Methods PPM Wag chemically modified by chlorosulfonic acid-pyfidine method and their bioactivities were compared.Results The substituting degree of S-PPM was 1.47.Results showed that S-PPM was more powerful in inhibiting the growth of tumor cell in vivo and in vitro and in promoting T,B lymphocytes than PPM.But there Wag no remarkable difference in promoting phagocytosis of macmphages.S-PPM was stronger in scavenging superoxide anion radical than PPM but it wag vice versa in scavenging hydroxyl radical.Conclusion S-PPM inhibited the cancer cell growth mainly through specific immunity.Sulfate of PPM influenced its quality of scavenging free radicals greatly.