CAJ | 학술논문

目的了解北京地区部分甲型H1N1流感病毒感染确诊急诊患者中人冠状病毒HCoV-OCA3、HCoV-229E、HCoV-NL63、HCoV-HKU1和人偏肺病毒(HMPV)的感染情况和临床表现特点.方法从2009-10～11期间北京地区部分确诊感染甲型H1NI流感病毒病例中,选择采集32份鼻咽拭子标本,利用荧光定量RT-PCR法进行HCoV-OC43、HCoV-229E、HCoV-NL63、HCoV-HKU1和HMPV筛查;对阳性患者进行临床表现初步分析.结果 32份鼻咽拭子标本中荧光定量RT-PCR检出4例(12.5%)HCoV-229E阳性,1例(3.1%)HCoV-HKU1阳性,2例(6.3%)HMPV阳性,未检测到HCoV-OCA3和HCoV-NL63阳性;检出阳性标本的临床表现为急性呼吸道症状,病情较轻,病程多为1周左右.结论 2009-10～11北京地区部分确诊的感染甲型H1N1流感病毒病例中存在有人冠状病毒或人偏肺病毒共感染的情况.
목적 료해북경지구부분갑형H1N1류감병독감염학진급진환자중인관상병독HCoV-OCA3、HCoV-229E、HCoV-NL63、HCoV-HKU1화인편폐병독(HMPV)적감염정황화림상표현특점.방법 종2009-10～11기간북경지구부분학진감염갑형H1NI류감병독병례중,선택채집32빈비인식자표본,이용형광정량RT-PCR법진행HCoV-OC43、HCoV-229E、HCoV-NL63、HCoV-HKU1화HMPV사사;대양성환자진행림상표현초보분석.결과 32빈비인식자표본중형광정량RT-PCR검출4례(12.5%)HCoV-229E양성,1례(3.1%)HCoV-HKU1양성,2례(6.3%)HMPV양성,미검측도HCoV-OCA3화HCoV-NL63양성;검출양성표본적림상표현위급성호흡도증상,병정교경,병정다위1주좌우.결론 2009-10～11북경지구부분학진적감염갑형H1N1류감병독병례중존재유인관상병독혹인편폐병독공감염적정황.
Objective To study the etiology and clinical symptoms associated with the detection of the human coronavirus (HCoVs) and human metapneumovirus (HMPV)in novel H1N1 infection adult patients of emergency department in Beijing .Methods 32 nasopharyngeal swab specimens were collected from H1N1 infection adult patients of Emergency Department in Beijing from October to November, 2009.We performed the screening of HcoV - 229E, HCoV - OCA3, HCoV - NL63, HCoV -HKU1 and HMPV by real - time RT - PCR.In the meantime, clinical data associated with HCoV -229E, HCoV -HKU1 and HMPV infection were examined retrospectively.Results 4(12.5%) specimens were positive for HCoV -229E, 1(3.1%) were positive for HCoV - HKU1 and 2(6.3%) were positive for HMPV by real - time RT - PCR.The main clinical manifestations were noted including fever, cough, rhinorrhea, nasal obstruction and sputum.Conclusion Our data show that co - infection with HCoV-229E, HCoV -HKU1 or HMPV in adult patients is a common event in H1N1 infection adult patients, but usually resulted in milder influenza- like illnesses.