农业科学与技术（英文版）
農業科學與技術（英文版）
농업과학여기술（영문판）
AGRICULTURAL SCIENCE & TECHNOLOGY
2010年
8期
129-132,136
,共5页
条斑紫菜%凝集素%提取%性质
條斑紫菜%凝集素%提取%性質
조반자채%응집소%제취%성질
Porphyra yezoensis%Lectin%Extraction%Properties
[目的]研究条斑紫菜(Porphyra yezoensis)凝集素的提取及分离纯化条件,并测定凝集素的性质.[方法]以比活力为指标,研究了缓冲体系、固液比、温度及时间等条件对条斑紫菜凝集素提取效果的影响.采用DEAE-Sepharose FF离子交换层析技术进行分离,确定了分离的最佳条件.以血凝集活性为指标,测定了凝集素的糖专一性、二价阳离子依赖性、温度及酸碱稳定性等性质.[结果]固液比为1∶ 5的Tris-HCl (25 mmol/L,pH值7.5)缓冲液在40 ℃条件下提取20 h得到的提取液具有最高的比活力.条斑紫菜凝集素在50 ℃条件下加热30 min后活性保持不变,表现出一定的热稳定性,最适作用pH值为7～9.该凝集素能够与麦芽糖、半乳糖、阿拉伯糖及木糖结合,其中与麦芽糖的结合最强,凝集活性依赖Ca2+、Mg2+等二价阳离子.[结论]该研究为阐明条斑紫菜的免疫机制及其高效综合利用提供了理论依据.
[目的]研究條斑紫菜(Porphyra yezoensis)凝集素的提取及分離純化條件,併測定凝集素的性質.[方法]以比活力為指標,研究瞭緩遲體繫、固液比、溫度及時間等條件對條斑紫菜凝集素提取效果的影響.採用DEAE-Sepharose FF離子交換層析技術進行分離,確定瞭分離的最佳條件.以血凝集活性為指標,測定瞭凝集素的糖專一性、二價暘離子依賴性、溫度及痠堿穩定性等性質.[結果]固液比為1∶ 5的Tris-HCl (25 mmol/L,pH值7.5)緩遲液在40 ℃條件下提取20 h得到的提取液具有最高的比活力.條斑紫菜凝集素在50 ℃條件下加熱30 min後活性保持不變,錶現齣一定的熱穩定性,最適作用pH值為7～9.該凝集素能夠與麥芽糖、半乳糖、阿拉伯糖及木糖結閤,其中與麥芽糖的結閤最彊,凝集活性依賴Ca2+、Mg2+等二價暘離子.[結論]該研究為闡明條斑紫菜的免疫機製及其高效綜閤利用提供瞭理論依據.
[목적]연구조반자채(Porphyra yezoensis)응집소적제취급분리순화조건,병측정응집소적성질.[방법]이비활력위지표,연구료완충체계、고액비、온도급시간등조건대조반자채응집소제취효과적영향.채용DEAE-Sepharose FF리자교환층석기술진행분리,학정료분리적최가조건.이혈응집활성위지표,측정료응집소적당전일성、이개양리자의뢰성、온도급산감은정성등성질.[결과]고액비위1∶ 5적Tris-HCl (25 mmol/L,pH치7.5)완충액재40 ℃조건하제취20 h득도적제취액구유최고적비활력.조반자채응집소재50 ℃조건하가열30 min후활성보지불변,표현출일정적열은정성,최괄작용pH치위7～9.해응집소능구여맥아당、반유당、아랍백당급목당결합,기중여맥아당적결합최강,응집활성의뢰Ca2+、Mg2+등이개양리자.[결론]해연구위천명조반자채적면역궤제급기고효종합이용제공료이론의거.
[Objective] The aim was to explore the extraction technique and purification method of lectin from Porpya yezoensis,as well as to investigate the properties of lectin. [Method] The effects of four factors such as buffer system,solid-liquid ratio,temperature,and extracting time on the lectin extraction result from P. yezoensis were investigated by the use of specific activity as the indicator. Then the lectin was purified by DEAE-Sepharose FF chromatography. The hemagglutination activity was used as the indicator,and the properties of the lectin such as carbohydrate specificity,divalent cations dependence,temperature,acidic and alkaline stability and others were detected. [Result] The optimal extraction conditions were: extraction ageat Tris-HCl of 40 ℃ ,and extracting time of 16 h. The activity of the lectin of P. yezoensis didn't change after 30 min of being heated at 50 ℃,which showed a certain extent of thermal stability,and the suitable pH value was 7-9. The lectin of P. yezoensis could combine with maltose,D-galactose,D-xylose and L-Arabinose,in which the binding force with the maltose was the strongest. The lectin activity was depended on the addition of Ca2+ and Mg2+. [Conclusion] The research had provided theoretical basis for the clarification of immune mechanism of P. yezoensis,as well as its high effective utilization.
