CAJ | 학술논문

以甜叶菊茎段为外植体、MS为基本培养基，研究了消毒剂70％酒精和0．1％升汞的不同时间处理组合的灭菌效果和对试管苗再生的影响，同时还探讨了不同种类、不同浓度植物生长调节剂对试管苗增殖、生根及移栽的影响。结果表明，70％酒精处理15-30s＋0．1％升汞处理4m较为适合茎段外植体灭菌，污染率低、苗再生率高；6-BA与NAA组合的试管苗增殖系数比KT、Ad与NAA组合要高，培养基中添加1．0mg／L6-BA＋0．1-0．2mg／LNAA植物生长调节剂组合适合试管苗增殖，增殖系数可达7．4；培养基中添加0．0-0．5mg／L的IBA或NAA均适合试管苗生根，生根的试管苗移栽成活率较高。
이첨협국경단위외식체、MS위기본배양기，연구료소독제70％주정화0．1％승홍적불동시간처리조합적멸균효과화대시관묘재생적영향，동시환탐토료불동충류、불동농도식물생장조절제대시관묘증식、생근급이재적영향。결과표명，70％주정처리15-30s＋0．1％승홍처리4m교위괄합경단외식체멸균，오염솔저、묘재생솔고；6-BA여NAA조합적시관묘증식계수비KT、Ad여NAA조합요고，배양기중첨가1．0mg／L6-BA＋0．1-0．2mg／LNAA식물생장조절제조합괄합시관묘증식，증식계수가체7．4；배양기중첨가0．0-0．5mg／L적IBA혹NAA균괄합시관묘생근，생근적시관묘이재성활솔교고。
The stem -sections were chosen as explants for plant tissue culture in the MS basic medium. The test investigated the influences of disinfectors 70% alcohol and 0.1% HgC12 on sterilizing effects and shoot regenera- tion at first. And then, this test studied the effects of the plant growth regulators on shoot proliferating, rooting and transplanting too. The results showed that 70% alcohol treated for 15 - 30s with 0.1% HgCI2 treated for 4m was suitable for explants sterilizing and got lower explants infectious ratio and higher shoot formation ratio. The combinations of 6 - BA and NAA got higher proliferation coefficient than the combinations of Ad and NAA or the combinations of KT and NAA. The basic MS medium with 1.0mg/L 6 - BA and 0.1 - 0.2mg/L NAA was suit- able for shoot proliferation, and the coefficient of proliferation could reach 7.4. The 1/2 MS medium with 0.0 - 0.5mg/L IBA or NAA was suitable for shoot rooting and the survival rate was high.