中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2010年
15期
1-4
,共4页
黄凯清%柯佩奇%梁立治%彭文明%彭娟%刘少颜
黃凱清%柯珮奇%樑立治%彭文明%彭娟%劉少顏
황개청%가패기%량립치%팽문명%팽연%류소안
卵巢肿瘤%基质金属蛋白酶9%胶原Ⅳ型%抗原,CD34
卵巢腫瘤%基質金屬蛋白酶9%膠原Ⅳ型%抗原,CD34
란소종류%기질금속단백매9%효원Ⅳ형%항원,CD34
Ovarian neoplasms%Matrix metalloproteinase 9%Collagen type IV%Antigens,CD34
目的 探讨基质金属蛋白酶(MMP)-9、Ⅳ型胶原、CD34在卵巢上皮性肿瘤中的表达及意义.方法 卵巢上皮性肿瘤患者82例,其中卵巢恶性上皮性肿瘤48例,卵巢交界性上皮性肿瘤23例,卵巢良性上皮性肿瘤11例.采用免疫组化染色方法,检测MMP-9、Ⅳ型胶原和CD34在肿瘤组织中的表达情况.结果 MMP-9的表达强度随肿瘤恶性程度增加而增强(F=39.306,P<0.01).CD34的表达亦随肿瘤恶性程度增加而增强[卵巢良性上皮性肿瘤为(17.18±5.64)%,卵巢交界性上皮性肿瘤为(29.76±7.18)%,高分化卵巢恶性上皮性肿瘤为(57.20±8.55)%,中分化卵巢恶性上皮性肿瘤为(71.20±8.48)%,低分化卵巢恶性上皮性肿瘤为(90.38 ±20.03)%](F=100.072,P<0.01).Ⅳ型胶原在卵巢恶性上皮性肿瘤中的表达强度低于在卵巢交界性上皮性肿瘤和卵巢良性上皮性肿瘤中的表达强度(F=11.554,P<0.01). MMP-9表达与CD34表达、Ⅳ型胶原缺失表达均呈正相关(r值分别为0.802和0.796,P<0.01).结论 MMP-9、CD34表达随着卵巢上皮性肿瘤恶性程度的增加而增强,Ⅳ型胶原的表达在卵巢恶性上皮性肿瘤中明显减少.MMP-9表达与CD34表达、Ⅳ型胶原缺失表达呈正相关.通过联合检测MMP-9、CD34、Ⅳ型胶原在卵巢上皮性肿瘤中的表达对判断其恶性程度具有重要的临床意义.
目的 探討基質金屬蛋白酶(MMP)-9、Ⅳ型膠原、CD34在卵巢上皮性腫瘤中的錶達及意義.方法 卵巢上皮性腫瘤患者82例,其中卵巢噁性上皮性腫瘤48例,卵巢交界性上皮性腫瘤23例,卵巢良性上皮性腫瘤11例.採用免疫組化染色方法,檢測MMP-9、Ⅳ型膠原和CD34在腫瘤組織中的錶達情況.結果 MMP-9的錶達彊度隨腫瘤噁性程度增加而增彊(F=39.306,P<0.01).CD34的錶達亦隨腫瘤噁性程度增加而增彊[卵巢良性上皮性腫瘤為(17.18±5.64)%,卵巢交界性上皮性腫瘤為(29.76±7.18)%,高分化卵巢噁性上皮性腫瘤為(57.20±8.55)%,中分化卵巢噁性上皮性腫瘤為(71.20±8.48)%,低分化卵巢噁性上皮性腫瘤為(90.38 ±20.03)%](F=100.072,P<0.01).Ⅳ型膠原在卵巢噁性上皮性腫瘤中的錶達彊度低于在卵巢交界性上皮性腫瘤和卵巢良性上皮性腫瘤中的錶達彊度(F=11.554,P<0.01). MMP-9錶達與CD34錶達、Ⅳ型膠原缺失錶達均呈正相關(r值分彆為0.802和0.796,P<0.01).結論 MMP-9、CD34錶達隨著卵巢上皮性腫瘤噁性程度的增加而增彊,Ⅳ型膠原的錶達在卵巢噁性上皮性腫瘤中明顯減少.MMP-9錶達與CD34錶達、Ⅳ型膠原缺失錶達呈正相關.通過聯閤檢測MMP-9、CD34、Ⅳ型膠原在卵巢上皮性腫瘤中的錶達對判斷其噁性程度具有重要的臨床意義.
목적 탐토기질금속단백매(MMP)-9、Ⅳ형효원、CD34재란소상피성종류중적표체급의의.방법 란소상피성종류환자82례,기중란소악성상피성종류48례,란소교계성상피성종류23례,란소량성상피성종류11례.채용면역조화염색방법,검측MMP-9、Ⅳ형효원화CD34재종류조직중적표체정황.결과 MMP-9적표체강도수종류악성정도증가이증강(F=39.306,P<0.01).CD34적표체역수종류악성정도증가이증강[란소량성상피성종류위(17.18±5.64)%,란소교계성상피성종류위(29.76±7.18)%,고분화란소악성상피성종류위(57.20±8.55)%,중분화란소악성상피성종류위(71.20±8.48)%,저분화란소악성상피성종류위(90.38 ±20.03)%](F=100.072,P<0.01).Ⅳ형효원재란소악성상피성종류중적표체강도저우재란소교계성상피성종류화란소량성상피성종류중적표체강도(F=11.554,P<0.01). MMP-9표체여CD34표체、Ⅳ형효원결실표체균정정상관(r치분별위0.802화0.796,P<0.01).결론 MMP-9、CD34표체수착란소상피성종류악성정도적증가이증강,Ⅳ형효원적표체재란소악성상피성종류중명현감소.MMP-9표체여CD34표체、Ⅳ형효원결실표체정정상관.통과연합검측MMP-9、CD34、Ⅳ형효원재란소상피성종류중적표체대판단기악성정도구유중요적림상의의.
Objective To explore the expression and significance of matrix metalloproteinase (MMP)-9, IV collagen and CD34 in epithelial ovarian tumor. Methods Eighty-two patients with epithelial ovarian tumor, among them,there were 48 malignant epithelial ovarian carcinomas, 23 borderline epithelial ovarian tumors and 11 benign epithelial ovarian tumors. The expression of MMP-9, IV collagen and CD34 were detected by immunohistochemistry. Results The expression of MMP-9 was strongly linked to the degree of malignant ovarian carcinomas (F= 39.306,P< 0.01). The expression of CD34 was also strongly linked to the degree of malignant ovarian carcinomas [benign epithelial ovarian tumors was (17.18±5.64)%,borderline epithelial ovarian tumors was (29.76±7.18)%,well-differentiated malignant epithelial ovarian carcinomas was (57.20±8.55)%,moderately-differentiated malignant epithelial ovarian carcinomas was (71.20±8.48)%, poorly-differentiated malignant epithelial ovarian carcinomas was(90.38±20.03)%](F= 100.072, P < 0.01). The expression of IV collagen in malignant epithelial ovarian carcinomas was different from that in borderline epithelial ovarian tumors and benign epithelial ovarian tumors (F = 11.554,P<0.0l). The expression of MMP-9 was positive correlation with the loss expression of IV collagen and the expression of CD34 (r=0.796,0.802,P< 0.01).Conclusions The positive expression of MMP-9,CD34 and the negative expression of IV collagen are obviously relevant to degree of malignant ovarian carcinomas.The combined testing on expression of MMP-9,CD34, IV collagen on ovarian carcinomas is significant to decide degree of malignant ovarian carcinomas and evaluate future development.
