CAJ | 학술논문

目的研究运动训练对血管性痴呆(VD)大鼠海马β-淀粉样多肽(Aβ)及β分泌酶(BACE)的影响.方法将30只SD大鼠数字随机表分为运动组,模型组和假手术组,采用结扎双侧颈总动脉法制做VD大鼠模型,术后4周后行水迷宫检测评估大鼠学习记忆能力.采用免疫组化技术检测大鼠海马Aβ,BACE表达.结果 Morris水迷宫实验中,模型组学习记能力下降,逃避潜伏期[分别为(101.34±19.67)s,(95.42±23.89)s,(89.39±22.67)s,(90.12±19.77)s]明显长于假手术组[对应为(62.13±11.38)s,(24.84±13.69)s,(16.98±12.51)s,(11.41±8.93)s] (P＜0.05),运动组较模型组学习记能力增强[对应为(80.15 ±21.56)s,(51.24±20.91)s,(43.78±22.36)s,(45.67±20.87)s],差异有统计学意义(P＜0.05).运动组大鼠海马Aβ灰度值(130.12±19.01),模型动组为(116.77±23.67),假手术组为(148.44±17.67),各组之间差异有统计学意义(P＜ 0.05).运动组大鼠海马BACE灰度值(131.21±25.25),模型组为(120.53±10.21),假手术组为(162.38 ±28.11),各组之间差异有统计学意义(P＜0.05).结论运动训练可下调VD大鼠海马BACE及Aβ的表达,进而改善VD大鼠学习记忆能力.
목적 연구운동훈련대혈관성치태(VD)대서해마β-정분양다태(Aβ)급β분비매(BACE)적영향.방법 장30지SD대서수자수궤표분위운동조,모형조화가수술조,채용결찰쌍측경총동맥법제주VD대서모형,술후4주후행수미궁검측평고대서학습기억능력.채용면역조화기술검측대서해마Aβ,BACE표체.결과 Morris수미궁실험중,모형조학습기능력하강,도피잠복기[분별위(101.34±19.67)s,(95.42±23.89)s,(89.39±22.67)s,(90.12±19.77)s]명현장우가수술조[대응위(62.13±11.38)s,(24.84±13.69)s,(16.98±12.51)s,(11.41±8.93)s] (P＜0.05),운동조교모형조학습기능력증강[대응위(80.15 ±21.56)s,(51.24±20.91)s,(43.78±22.36)s,(45.67±20.87)s],차이유통계학의의(P＜0.05).운동조대서해마Aβ회도치(130.12±19.01),모형동조위(116.77±23.67),가수술조위(148.44±17.67),각조지간차이유통계학의의(P＜ 0.05).운동조대서해마BACE회도치(131.21±25.25),모형조위(120.53±10.21),가수술조위(162.38 ±28.11),각조지간차이유통계학의의(P＜0.05).결론 운동훈련가하조VD대서해마BACE급Aβ적표체,진이개선VD대서학습기억능력.
Objective To study the effect of exercise training on β-amyloid polypeptide (Aβ) and β-secretase(BACE) in the hippocampus of the rats with vascular dementia (VD).Methods 30 Sprague-Dawley (SD) rats were carried out to an exercise group (n =10 ),a model group (n =10 ),and a sham-operation group ( n =10 ).VD rat models were made by the ligation of bilateral common carotid arteries.Morris water maze test were carried out 4 weeks after the operation to assess the ability in learning and memory of the rats and Aβ and β-secretase (BACE) expression was detected in the hippocampus of the rats using immunohistochemical techniques.Results In the Morris water maze test,the model group showed reduction in the learning and memorizing ability,with obvious longer escape latencies ( ( 101.34 ± 19.67 ) s,(95.42 ± 23.89 ) s,( 89.39 ± 22.67 ) s,( 90.12 ± 19.77 ) s,respective-ly) than that of sham-operation group ( ( 62.13 ± 11.38 ) s,( 24.84 ± 13.69 ) s,( 16.98 ± 12.51 )s,( 11.41 ± 8.93 ) s,correspond-dingly) (P ＜ 0.05 ),and the exercise group was improved in the learning and memorizing ability ( corresponding to ( 80.15 ± 21.56 ) s,( 51.24 ± 20.91 ) s,( 43.78 ± 22.36) s,( 45.67 ± 20.87 ) s ),compared with the model group(P＜0.05).The grey values of Aβ in the hippocampus of the rats for the exercise group was ( 130.12 ± 19.01 ),( 116.77 ± 23.67 ) for the model group and ( 148.44 ± 17.67 ) for the sham-operation group(P＜ 0.05).The grey values of BACE in the hippocampus of the ratsfor the exercise group were( 131.21± 25.25 ),( 120.53± 10.21 ) for the model group(P＜ 0.05 ) and ( 162.38 ± 28.11 ) for the sham-operation group (P ＜ 0.05).Conclusion Exercise training can lower the expression of BACE and Aβ in the hippocampus of rats with VD,therefore improving the learning and memory ability of rats with VD.