CAJ | 학술논문

目的观察异种脱蛋白骨(DPB)复合皮质骨来源成骨细胞修复兔桡骨临界骨缺损的效果.方法 36只新西兰大白兔的桡骨建立1.5 cm临界骨缺损模型,随机分为A、B、C3组,分别植入:A组,异种脱蛋白骨与成骨细胞复合物；B组,自体髂骨；C组,异种脱蛋白骨.术后12周检测如下:X线观察、骨密度测量、标本大体观察、生物力学测试、组织学观察.结果 X线显示:A、B两组均可见植入体与桡骨融合,骨折线消失,C组成骨量少,骨折线仍存在.骨密度测量:A、B两组差异无统计学意义(F=2.388,P＞0.05)；A、C两组的差异有统计学意义(F=10.869,P＜0.05).标本大体观察:A、B两组骨折线消失；C组骨折线存在.生物力学测试:A、B组差异无统计学意义(F=1.353,P＞0.05)；A、C两组差异有统计学意义(F=4.395,P＜0.05).组织学观察:A组大量新生骨,B组被周围骨爬行替代,C组少量骨形成.结论异种脱蛋白骨复合成骨细胞可以高效修复兔桡骨临界骨缺损.
목적 관찰이충탈단백골(DPB)복합피질골래원성골세포수복토뇨골림계골결손적효과.방법 36지신서란대백토적뇨골건립1.5 cm림계골결손모형,수궤분위A、B、C3조,분별식입:A조,이충탈단백골여성골세포복합물；B조,자체가골；C조,이충탈단백골.술후12주검측여하:X선관찰、골밀도측량、표본대체관찰、생물역학측시、조직학관찰.결과 X선현시:A、B량조균가견식입체여뇨골융합,골절선소실,C조성골량소,골절선잉존재.골밀도측량:A、B량조차이무통계학의의(F=2.388,P＞0.05)；A、C량조적차이유통계학의의(F=10.869,P＜0.05).표본대체관찰:A、B량조골절선소실；C조골절선존재.생물역학측시:A、B조차이무통계학의의(F=1.353,P＞0.05)；A、C량조차이유통계학의의(F=4.395,P＜0.05).조직학관찰:A조대량신생골,B조피주위골파행체대,C조소량골형성.결론 이충탈단백골복합성골세포가이고효수복토뇨골림계골결손.
Objective To evaluate the efficiency of xenogenic deproteinized bone (DPB) based on cortical bone-derived osteoblasts as an alternative in repair of critical bone defect.Methods A total of 36 Newzealand rabbits were randomly divided into groups A,B and C.Critical bone defect with a length of 1.5 cm was created in one side of the radius.The defect was filled with a DPB scaffold seeded with osteoblasts (group A),autograft (group B) and DPB scaffold (group C).All specimens were selected for X-ray examination,bone mineral density (BMD) measurements,gross samples observation,biomechanical test and histological observation at 12th week postoperation.Results X-ray examination displayed that the radial defect which was still obvious in group C disappeared in groups A and B.BMD measurements showed that there was no significant difference in BMD between groups A and B,but that was higher in group C (P ＜ 0.05).Gross samples observation revealed that the radial defect which was still obvious in group C disappeared in groups A and B.Biomechanical testing demonstrated that the maximum bend load in groups A and B was significantly higher than in group C (P ＜ O.05 ).Histologically,there was abundant of newly bone formation in group A,the medullary cavity recanalized in group B,and a small amount of newly bone formation in group C.Conclusion Radial critical bone defect could be repaired efficiently by xenogenic DPB based on cortical bone-derived osteoblasts.