中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2010年
2期
159-160
,共2页
张宁%郭子进%白忠彬%曹大勇%丁睿%窦科峰
張寧%郭子進%白忠彬%曹大勇%丁睿%竇科峰
장저%곽자진%백충빈%조대용%정예%두과봉
癌,肝细胞%多药耐药%机制
癌,肝細胞%多藥耐藥%機製
암,간세포%다약내약%궤제
Carcinoma,hepatocellular%Multidrug resistance%Mechanism
目的 观察肝癌细胞株Li-7中边缘群在肝癌耐药中的作用,并分析其可能机制.方法 流式细胞术分选边缘群和主群细胞后,MTS法比较阿霉素作用下两亚群细胞增殖能力,流式分析ABCG2/BCRP表达,观察边缘群细胞的耐药作用.利用P13K抑制剂LY294002,分析Akt信号通路对边缘群细胞表型及耐药现象的作用.结果 边缘群细胞占Li-7细胞总数(2.07±0.22)%,阿霉素作用后其增殖能力高于主群细胞,吸光度值第9天(0.33±0.06比0.05±0.02,P<0.05),第12天(0.58±0.09比0.04±0.02,P<0.01),ABCG2/BCRP荧光强度高于主群细胞(212.72±21.01比96.36±18.36,P<0.05).LY294002作用后,边缘群细胞比例降至(0.96±0.21)%,阿霉素作用后细胞存活率由(64.93±6.96)%降至(38.73±5.25)%(P<0.01).结论 Li-7细胞边缘群耐药性较强,其机制与有功能的ABCG2/BCRP及Akt信号通路有关.
目的 觀察肝癌細胞株Li-7中邊緣群在肝癌耐藥中的作用,併分析其可能機製.方法 流式細胞術分選邊緣群和主群細胞後,MTS法比較阿黴素作用下兩亞群細胞增殖能力,流式分析ABCG2/BCRP錶達,觀察邊緣群細胞的耐藥作用.利用P13K抑製劑LY294002,分析Akt信號通路對邊緣群細胞錶型及耐藥現象的作用.結果 邊緣群細胞佔Li-7細胞總數(2.07±0.22)%,阿黴素作用後其增殖能力高于主群細胞,吸光度值第9天(0.33±0.06比0.05±0.02,P<0.05),第12天(0.58±0.09比0.04±0.02,P<0.01),ABCG2/BCRP熒光彊度高于主群細胞(212.72±21.01比96.36±18.36,P<0.05).LY294002作用後,邊緣群細胞比例降至(0.96±0.21)%,阿黴素作用後細胞存活率由(64.93±6.96)%降至(38.73±5.25)%(P<0.01).結論 Li-7細胞邊緣群耐藥性較彊,其機製與有功能的ABCG2/BCRP及Akt信號通路有關.
목적 관찰간암세포주Li-7중변연군재간암내약중적작용,병분석기가능궤제.방법 류식세포술분선변연군화주군세포후,MTS법비교아매소작용하량아군세포증식능력,류식분석ABCG2/BCRP표체,관찰변연군세포적내약작용.이용P13K억제제LY294002,분석Akt신호통로대변연군세포표형급내약현상적작용.결과 변연군세포점Li-7세포총수(2.07±0.22)%,아매소작용후기증식능력고우주군세포,흡광도치제9천(0.33±0.06비0.05±0.02,P<0.05),제12천(0.58±0.09비0.04±0.02,P<0.01),ABCG2/BCRP형광강도고우주군세포(212.72±21.01비96.36±18.36,P<0.05).LY294002작용후,변연군세포비례강지(0.96±0.21)%,아매소작용후세포존활솔유(64.93±6.96)%강지(38.73±5.25)%(P<0.01).결론 Li-7세포변연군내약성교강,기궤제여유공능적ABCG2/BCRP급Akt신호통로유관.
Objective To explore the effect and the mechanism of side population of hepatoma cell line Li-7 in chemoresistance. Methods Side population and main population cells were sorted by flow cytometer. MTS based Doxombicin resistant assay and ABCG2/BCRP expression assay were performed to show the chemoresistance of side population cells. The modulation of side population phenotype and its chemoresistance by Akt signaling were analyzed through the effects of PI3K inhibitor LY294002. Results (2.07±0.22) % cells in Li-7 cell line were side population. They survived better than main population cells with the treatment of Doxorubicin. The absorbance was (0.33±0.06 vs 0.05 +0.02 ,P<0.05) at day 9 and (0.58±0.09 vs 0.04±0.02, P<0.01 ) at day 12. The ABCG2/BCRP expression was higher in side population, and the fluorescence intensity was (212.72±21.01 vs 96.36±18.36, P<0.05). With the treatment of LY294002, the side population percentage was decreased to (0.96±0.21)%, and their chemoresistance of Doxorubicin partly disappeared. The cells viability was decreased from (64.93±6.96)% to (38.73±5.25)% ,P<0.01. Conclusion Side population cells contained higher ability of chemoresis-tance, which might be closely related to Akt signaling.
