中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2012年
9期
873-875
,共3页
抽动障碍%动物模型%SD大鼠%刻板行为
抽動障礙%動物模型%SD大鼠%刻闆行為
추동장애%동물모형%SD대서%각판행위
Tic disorder%Animal model%SD rat%Stereotypy
目的 观察阿扑吗啡(APO)和亚氨基二丙腈(IDPN)诱导的抽动障碍(TD)模型大鼠的刻板运动和头动次数,挑选可以全面体现TD特征性行为变化的造模方法. 方法 SD大鼠30只按随机数字表法分为正常组、APO组、IDPN组,每组10只.APO组大鼠给予APO 2 mg/kg腹腔注射,IDPN组大鼠给予IDPN 150 mg/kg腹腔注射,正常组给予生理盐水腹腔注射,给药体积均为1mL/100 g、每天1次、连用7d,注射结束5 min后记录各组大鼠5～10 min、15～20 min、25～30 min、35～40 min、45～50 min、55～60 min时间段的刻板行为评分和5 min内大鼠头部抽动次数. 结果 IDPN组和APO组的大鼠头动次数明显比正常组多,IDPN组大鼠的头动次数多于APO组,差异均有统计学意义(P＜0.05);APO组大鼠在5～10 min、15～20 min、25～30 min、35～40 min时间段刻板行为评分高于正常组,IDPN组大鼠各个时段的刻板行为学评分均高于正常组,IDPN组大鼠在45～50min和55～60 min时间段刻板行为评分高于APO组,差异均有统计学意义(P＜0.05). 结论 IDPN诱导的TD模型能比较全面的再现TD的特征性行为变化,是较理想的动物模型.
目的 觀察阿撲嗎啡(APO)和亞氨基二丙腈(IDPN)誘導的抽動障礙(TD)模型大鼠的刻闆運動和頭動次數,挑選可以全麵體現TD特徵性行為變化的造模方法. 方法 SD大鼠30隻按隨機數字錶法分為正常組、APO組、IDPN組,每組10隻.APO組大鼠給予APO 2 mg/kg腹腔註射,IDPN組大鼠給予IDPN 150 mg/kg腹腔註射,正常組給予生理鹽水腹腔註射,給藥體積均為1mL/100 g、每天1次、連用7d,註射結束5 min後記錄各組大鼠5～10 min、15～20 min、25～30 min、35～40 min、45～50 min、55～60 min時間段的刻闆行為評分和5 min內大鼠頭部抽動次數. 結果 IDPN組和APO組的大鼠頭動次數明顯比正常組多,IDPN組大鼠的頭動次數多于APO組,差異均有統計學意義(P＜0.05);APO組大鼠在5～10 min、15～20 min、25～30 min、35～40 min時間段刻闆行為評分高于正常組,IDPN組大鼠各箇時段的刻闆行為學評分均高于正常組,IDPN組大鼠在45～50min和55～60 min時間段刻闆行為評分高于APO組,差異均有統計學意義(P＜0.05). 結論 IDPN誘導的TD模型能比較全麵的再現TD的特徵性行為變化,是較理想的動物模型.
목적 관찰아복마배(APO)화아안기이병정(IDPN)유도적추동장애(TD)모형대서적각판운동화두동차수,도선가이전면체현TD특정성행위변화적조모방법. 방법 SD대서30지안수궤수자표법분위정상조、APO조、IDPN조,매조10지.APO조대서급여APO 2 mg/kg복강주사,IDPN조대서급여IDPN 150 mg/kg복강주사,정상조급여생리염수복강주사,급약체적균위1mL/100 g、매천1차、련용7d,주사결속5 min후기록각조대서5～10 min、15～20 min、25～30 min、35～40 min、45～50 min、55～60 min시간단적각판행위평분화5 min내대서두부추동차수. 결과 IDPN조화APO조적대서두동차수명현비정상조다,IDPN조대서적두동차수다우APO조,차이균유통계학의의(P＜0.05);APO조대서재5～10 min、15～20 min、25～30 min、35～40 min시간단각판행위평분고우정상조,IDPN조대서각개시단적각판행위학평분균고우정상조,IDPN조대서재45～50min화55～60 min시간단각판행위평분고우APO조,차이균유통계학의의(P＜0.05). 결론 IDPN유도적TD모형능비교전면적재현TD적특정성행위변화,시교이상적동물모형.
Objective To observe the stereotypy and the movement of head in rat models of tic disorder (TD) induced by apomorphine (APO) or iminodipropionitrile (IDPN),and select the best method for making rat models fully reflecting the characteristic behavior change. Methods A total of 30 male SD rats weighing 100-110 g were randomly divided into APO inducement group, IDPN inducement group and control group (n=10).The rats in the APO inducement group were intraperitoneally injected 2 mg/kg APO, and those in the IDPN inducement group were intraperitoneally injected 150 mg/kg IDPN; equal volume of saline was injected into the rats of the control group; treatments were given daily for a consecutive 7 d at a dosing volume of 1 mL/100 g. The stereotypy scale scores were observed and recorded at 5-10 min,15-20 min,25-30 min,35-40 min,45-50 min and 55-60 min after the treatment.Movement of head was noted within 5 min of sucess model making. Results The head move number of the rats in the APO and IDPN inducement groups was significantly larger than that in the control group (P＜0.05); the head move number of the rats in the IDPN inducement group was obviously larger than that in the APO inducement group (P＜0.05).The stereotyped behavior scores of rats in the APO inducement group were significantly higher than those in the control group at 5-10 min,15-20 min,25-30 min,and 35-40 min after treatment (P＜0.05); those of rats in the IDPN inducement group were higher than those in the control group at every time interval (P＜0.05); those in the IDPN inducement group were higher than those in the APO inducement group at 45-50 min and 55-60 min after tre atment (P＜0.05).Conclusion The behavioral changes of TD models induced by IDPN are more obvious than those of models induced by APO; TD model induced by IDPN is an ideal animal model for observing tic disorder.
