中华眼科杂志
中華眼科雜誌
중화안과잡지
Chinese Journal of Ophthalmology
2012年
9期
861-864
,共4页
细胞,培养的%培养基,无血清%肿瘤干细胞
細胞,培養的%培養基,無血清%腫瘤榦細胞
세포,배양적%배양기,무혈청%종류간세포
Cells,cultured%Culture media,serum-free%Neoplastic stem cells
肿瘤干细胞的分离和鉴定是肿瘤干细胞研究的关键.目前已利用无血清悬浮培养法从视网膜母细胞瘤、脉络膜黑色素瘤、乳腺癌、肺癌、结肠癌等多种肿瘤中成功分离、富集出肿瘤干细胞,使这些肿瘤干细胞的生物学功能和特性逐渐被认识.本文从无血清培养基的构成及其在眼部和部分全身肿瘤干细胞的分离培养中的应用方面进行综述,以期为眼部肿瘤干细胞的进一步研究提供依据.
腫瘤榦細胞的分離和鑒定是腫瘤榦細胞研究的關鍵.目前已利用無血清懸浮培養法從視網膜母細胞瘤、脈絡膜黑色素瘤、乳腺癌、肺癌、結腸癌等多種腫瘤中成功分離、富集齣腫瘤榦細胞,使這些腫瘤榦細胞的生物學功能和特性逐漸被認識.本文從無血清培養基的構成及其在眼部和部分全身腫瘤榦細胞的分離培養中的應用方麵進行綜述,以期為眼部腫瘤榦細胞的進一步研究提供依據.
종류간세포적분리화감정시종류간세포연구적관건.목전이이용무혈청현부배양법종시망막모세포류、맥락막흑색소류、유선암、폐암、결장암등다충종류중성공분리、부집출종류간세포,사저사종류간세포적생물학공능화특성축점피인식.본문종무혈청배양기적구성급기재안부화부분전신종류간세포적분리배양중적응용방면진행종술,이기위안부종류간세포적진일보연구제공의거.
The isolation and identification of cancer stem cells are the key points in exploring characteristics of cancer stem cells at present.Several species of cancer stem cells,for instance,retinoblastoma tumor stem cells,cancer stem cells of melanoma of choroid,breast cancer stem cells,lung cancer stem cells,colon cancer stem cells,etc.,have been isolated and cultured successfully by serum free medium while their biological functions and characteristics are acquired.This review focus on the application of serum free medium in the research of isolating cancer stem cells,both ocular and general,in terms of providing foundation for further research on ocular cancer stem cells.
