CAJ | 학술논문

目的观察经心肌梗死边缘区移植骨髓间充质干细胞(MSCs)24 h和2周后的全身分布.方法雄性SD大鼠的MSCs用溴脱氧尿苷(BrdU)标记.将雌性SD大鼠经心梗3周后随机分为2组.第1组为标记细胞(3×10,50μl)经心肌注入心梗边缘区(n=12).第2组为等体积的磷酸盐缓冲液(PBS)经心肌注入心梗边缘区(n=8).移植细胞24 h及2周后,体内细胞的分布情况通过实时定量逆转录-聚合酶链反应(RT-PCR)检测及病理切片免疫组织化学染色来评价.结果细胞经心梗边缘区移植后可以分布在心脏以及心外脏器脾脏、肺脏和肝脏中.移植24 h后细胞主要在心脏中分布(467 467±191 387),其他脏器中的分布较少,而在2周后移植细胞在包括心脏(112 388±43 751)在内的各个脏器中的分布都很少,病理切片免疫组织化学染色结果显示与RT-PCR结果相符.结论 MSCs经心梗边缘区移植后主要在心脏中分布,同时脾脏、肺脏、肝脏中也有分布,但其分布数量随着移植时间锐减.
목적 관찰경심기경사변연구이식골수간충질간세포(MSCs)24 h화2주후적전신분포.방법 웅성SD대서적MSCs용추탈양뇨감(BrdU)표기.장자성SD대서경심경3주후수궤분위2조.제1조위표기세포(3×10,50μl)경심기주입심경변연구(n=12).제2조위등체적적린산염완충액(PBS)경심기주입심경변연구(n=8).이식세포24 h급2주후,체내세포적분포정황통과실시정량역전록-취합매련반응(RT-PCR)검측급병리절편면역조직화학염색래평개.결과 세포경심경변연구이식후가이분포재심장이급심외장기비장、폐장화간장중.이식24 h후세포주요재심장중분포(467 467±191 387),기타장기중적분포교소,이재2주후이식세포재포괄심장(112 388±43 751)재내적각개장기중적분포도흔소,병리절편면역조직화학염색결과현시여RT-PCR결과상부.결론 MSCs경심경변연구이식후주요재심장중분포,동시비장、폐장、간장중야유분포,단기분포수량수착이식시간예감.
Objective To study the systemic distribution of bone marrow derived mesenchymal stem cells (MSCs) 24 h and 2 weeks after cell injection into the border zone of myocardial infarction area. Methods MSCs from male SD rats were labeled with Bromodeoxyuridine (BrdU). Three weeks after in-duction of myocardial infarction,female SD rats were randomized into 2 groups. Labeled cells (3 × 10 ,50 μl) were injected into the border zone of infarcted area in the one group (n = 12) ,and PBS of equal vol-ume was injected into the border zone of infarcted area in the other group ( n = 8 ). The systemic distribu-tion of MSCs was evaluated through real-time PCR and immunohistochemistry at time points of 24 h and 2 weeks after injection, respectively. Results Cells injected into border zone of infarcted area were distribu-ted to extra-cardiac organs such as spleen,lung and liver. Twenty-four h after injection,cells mainly con-centrated in the heart (467 467 ± 191 387) ,while obvious cell loss was noted in all organs including the heart ( 112 388 ±43 751 ) 2 weeks after injection. Of immunostaining were consistent with those of real-time PCR. Conclusion After injected into the border zone of infarcted area, MSCs mainly gathered in the heart with distributions into spleen, lung, and liver. However, substantial number of cells lost with pro-longed time span.