中华医学美学美容杂志
中華醫學美學美容雜誌
중화의학미학미용잡지
CHINESE JOURNAL OF MEDICAL AESTHETICS AND COSMETOLOGY
2010年
3期
183-186
,共4页
郑跃%赖维%万苗坚%陆春%区凤仙%杨素莲%易金玲%李美荣%叶聪秀
鄭躍%賴維%萬苗堅%陸春%區鳳仙%楊素蓮%易金玲%李美榮%葉聰秀
정약%뢰유%만묘견%륙춘%구봉선%양소련%역금령%리미영%협총수
光老化%组织蛋白酶G%皮肤%成纤维细胞
光老化%組織蛋白酶G%皮膚%成纖維細胞
광노화%조직단백매G%피부%성섬유세포
Photoaging%Cathepsin G%Skin%Fibroblast
目的 探讨组织蛋白酶G(cathepsin G)在光老化皮肤中的表达变化及意义.方法 培养原代人皮肤成纤维细胞,8-甲氧补骨脂素+长波紫外线(8-MOP+UVA)法体外诱导培养细胞光老化.衰老相关-β半乳糖苷酶(SA-β-Gal)染色证明老化诱导成功.蛋白印迹技术及反转录-聚合酶链式反应(RT-PCR)对比检测光老化成纤维细胞及正常成纤维细胞组织蛋白酶G蛋白及基因表达.结果 Western印迹法结果 示光老化诱导组组织蛋白酶G表达上调为单UVA诱导组、单8-MOP孵育组及空白组的(1.70±0.028)倍.实时RT-PCR结果 示光老化细胞组织蛋白酶G mRNA表达上调为空白组的(1.42±0.09)倍(P<0.01).结论 组织蛋白酶G在光老化成纤维细胞中高表达,可能通过改变细胞外基质成分和激活基质金属蛋白酶参与皮肤光老化所致.
目的 探討組織蛋白酶G(cathepsin G)在光老化皮膚中的錶達變化及意義.方法 培養原代人皮膚成纖維細胞,8-甲氧補骨脂素+長波紫外線(8-MOP+UVA)法體外誘導培養細胞光老化.衰老相關-β半乳糖苷酶(SA-β-Gal)染色證明老化誘導成功.蛋白印跡技術及反轉錄-聚閤酶鏈式反應(RT-PCR)對比檢測光老化成纖維細胞及正常成纖維細胞組織蛋白酶G蛋白及基因錶達.結果 Western印跡法結果 示光老化誘導組組織蛋白酶G錶達上調為單UVA誘導組、單8-MOP孵育組及空白組的(1.70±0.028)倍.實時RT-PCR結果 示光老化細胞組織蛋白酶G mRNA錶達上調為空白組的(1.42±0.09)倍(P<0.01).結論 組織蛋白酶G在光老化成纖維細胞中高錶達,可能通過改變細胞外基質成分和激活基質金屬蛋白酶參與皮膚光老化所緻.
목적 탐토조직단백매G(cathepsin G)재광노화피부중적표체변화급의의.방법 배양원대인피부성섬유세포,8-갑양보골지소+장파자외선(8-MOP+UVA)법체외유도배양세포광노화.쇠로상관-β반유당감매(SA-β-Gal)염색증명노화유도성공.단백인적기술급반전록-취합매련식반응(RT-PCR)대비검측광노화성섬유세포급정상성섬유세포조직단백매G단백급기인표체.결과 Western인적법결과 시광노화유도조조직단백매G표체상조위단UVA유도조、단8-MOP부육조급공백조적(1.70±0.028)배.실시RT-PCR결과 시광노화세포조직단백매G mRNA표체상조위공백조적(1.42±0.09)배(P<0.01).결론 조직단백매G재광노화성섬유세포중고표체,가능통과개변세포외기질성분화격활기질금속단백매삼여피부광노화소치.
Objective To investigate the role of cathepsin G in photoaged fibroblasts. Methods Human fibroblasts were cultured and induced to premature senescence using UVA + MOP methods. Senescence-associated-β-galactosidase (SA-β-gal) stain was used to evaluate the positive rate of aged cells. The mRNA and protein expression of cathepsin G in photoaged fibroblasts were detected by real-time RT-PCR and Western blot techniques. Results Over 98 % induced cells presented a positive SA-β-gal straining. The expression of cathepsin G, detected by Western blot, was increased to (1. 70±0. 028) times of the control. And RT-PCR revealed that the synthesis of cathepsin G mRNA was also up-regulated to 1. 42±0. 09. Conclusion The results of our study demonstrates a significant correlation between photoaged fibroblasts and cathepsin G. The up-regulation of cathepsin G may play an important role in the damages of extracellular matrix and activation of MMPS in photoaged human skin.
