中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2011年
1期
37-41
,共5页
马丽娟%李亦工%黄菱%韩梅%马百菁%孙伯坚%林佳静%宋婷阁
馬麗娟%李亦工%黃蔆%韓梅%馬百菁%孫伯堅%林佳靜%宋婷閣
마려연%리역공%황릉%한매%마백정%손백견%림가정%송정각
胃肿瘤%肿瘤转移%赖氨酰氧化酶%基质金属蛋白酶2
胃腫瘤%腫瘤轉移%賴氨酰氧化酶%基質金屬蛋白酶2
위종류%종류전이%뢰안선양화매%기질금속단백매2
Stomach neoplasms%Neoplasm metastasis%Lysyl oxidase%Matrix metallo-proteinases-2
目的 探讨赖氨酰氧化酶(LOX)和基质金属蛋白酶2(MMP-2)在胃癌组织中的表达及其与胃癌转移的关系.方法 收集手术切除新鲜胃癌及相应的癌旁胃组织,用逆转录聚合酶链反应(RT-PCR)方法检测LOX mRNA和MMP-2 mRNA表达,Western blot方法检测LOX蛋白和MMP-2蛋白表达.结果 LOX mRNA在胃癌组织和癌旁胃组织中的相对表达量分别为0.5328±0.1367和0.2436±0.1372(P<0.01),MMP-2 mRNA在胃癌组织和癌旁胃组织中的相对表达量分别为0.7980±0.1571和0.3231±0.1672(P<0.05);LOX mRNA在有淋巴结转移和无淋巴结转移的胃癌组织中的相对表达量分别为0.6336±0.1547和0.4914±0.1408,MMP-2 mRNA在有淋巴结转移和无淋巴结转移的胃癌组织中的相对表达量分别为0.8762±0.1381和0.6362±0.1936,差异均有统计学意义(均P<0.05).LOX蛋白在胃癌组织和癌旁胃组织中的相对表达量分别为0.5237±0.1426和0.2972±0.1480(P<0.05),在有淋巴结转移和无淋巴结转移的胃癌组织中相对表达量分别为0.6880±0.1263和0.4944±0.1217(P<0.05);MMP-2蛋白在胃癌组织和癌旁胃组织中的相对表达量分别为0.7365±0.1607和0.3321±0.1480(P<0.05),在有淋巴结转移和无淋巴结转移的胃癌组织中相对表达量分别为0.8097±0.1632和0.5132土0.1366(P<0.05).胃癌组织中,LOX mRNA与MMP-2 mRNA、LOX蛋白与MMP-2蛋白相对表达量呈正相关(r=0.873,P<0.001;r=0.881,P<0.001);在伴有淋巴结转移的胃癌组织中,LOX mRNA与MMP-2 mRNA、LOX蛋白与MMP-2蛋白相对表达量也呈正相关(r=0.923,P<0.001;r=0.972,P<0.001).结论 LOX和MMP-2在胃癌组织中的表达明显高于相应的癌旁胃组织;在伴有淋巴结转移的胃癌组织中,LOX和MMP-2表达高于无淋巴结转移的胃癌组织,且LOX和MMP-2表达呈正相关,提示LOX和MMP-2对胃癌的发生和转移有促进作用,且两者可能有协同作用.
目的 探討賴氨酰氧化酶(LOX)和基質金屬蛋白酶2(MMP-2)在胃癌組織中的錶達及其與胃癌轉移的關繫.方法 收集手術切除新鮮胃癌及相應的癌徬胃組織,用逆轉錄聚閤酶鏈反應(RT-PCR)方法檢測LOX mRNA和MMP-2 mRNA錶達,Western blot方法檢測LOX蛋白和MMP-2蛋白錶達.結果 LOX mRNA在胃癌組織和癌徬胃組織中的相對錶達量分彆為0.5328±0.1367和0.2436±0.1372(P<0.01),MMP-2 mRNA在胃癌組織和癌徬胃組織中的相對錶達量分彆為0.7980±0.1571和0.3231±0.1672(P<0.05);LOX mRNA在有淋巴結轉移和無淋巴結轉移的胃癌組織中的相對錶達量分彆為0.6336±0.1547和0.4914±0.1408,MMP-2 mRNA在有淋巴結轉移和無淋巴結轉移的胃癌組織中的相對錶達量分彆為0.8762±0.1381和0.6362±0.1936,差異均有統計學意義(均P<0.05).LOX蛋白在胃癌組織和癌徬胃組織中的相對錶達量分彆為0.5237±0.1426和0.2972±0.1480(P<0.05),在有淋巴結轉移和無淋巴結轉移的胃癌組織中相對錶達量分彆為0.6880±0.1263和0.4944±0.1217(P<0.05);MMP-2蛋白在胃癌組織和癌徬胃組織中的相對錶達量分彆為0.7365±0.1607和0.3321±0.1480(P<0.05),在有淋巴結轉移和無淋巴結轉移的胃癌組織中相對錶達量分彆為0.8097±0.1632和0.5132土0.1366(P<0.05).胃癌組織中,LOX mRNA與MMP-2 mRNA、LOX蛋白與MMP-2蛋白相對錶達量呈正相關(r=0.873,P<0.001;r=0.881,P<0.001);在伴有淋巴結轉移的胃癌組織中,LOX mRNA與MMP-2 mRNA、LOX蛋白與MMP-2蛋白相對錶達量也呈正相關(r=0.923,P<0.001;r=0.972,P<0.001).結論 LOX和MMP-2在胃癌組織中的錶達明顯高于相應的癌徬胃組織;在伴有淋巴結轉移的胃癌組織中,LOX和MMP-2錶達高于無淋巴結轉移的胃癌組織,且LOX和MMP-2錶達呈正相關,提示LOX和MMP-2對胃癌的髮生和轉移有促進作用,且兩者可能有協同作用.
목적 탐토뢰안선양화매(LOX)화기질금속단백매2(MMP-2)재위암조직중적표체급기여위암전이적관계.방법 수집수술절제신선위암급상응적암방위조직,용역전록취합매련반응(RT-PCR)방법검측LOX mRNA화MMP-2 mRNA표체,Western blot방법검측LOX단백화MMP-2단백표체.결과 LOX mRNA재위암조직화암방위조직중적상대표체량분별위0.5328±0.1367화0.2436±0.1372(P<0.01),MMP-2 mRNA재위암조직화암방위조직중적상대표체량분별위0.7980±0.1571화0.3231±0.1672(P<0.05);LOX mRNA재유림파결전이화무림파결전이적위암조직중적상대표체량분별위0.6336±0.1547화0.4914±0.1408,MMP-2 mRNA재유림파결전이화무림파결전이적위암조직중적상대표체량분별위0.8762±0.1381화0.6362±0.1936,차이균유통계학의의(균P<0.05).LOX단백재위암조직화암방위조직중적상대표체량분별위0.5237±0.1426화0.2972±0.1480(P<0.05),재유림파결전이화무림파결전이적위암조직중상대표체량분별위0.6880±0.1263화0.4944±0.1217(P<0.05);MMP-2단백재위암조직화암방위조직중적상대표체량분별위0.7365±0.1607화0.3321±0.1480(P<0.05),재유림파결전이화무림파결전이적위암조직중상대표체량분별위0.8097±0.1632화0.5132토0.1366(P<0.05).위암조직중,LOX mRNA여MMP-2 mRNA、LOX단백여MMP-2단백상대표체량정정상관(r=0.873,P<0.001;r=0.881,P<0.001);재반유림파결전이적위암조직중,LOX mRNA여MMP-2 mRNA、LOX단백여MMP-2단백상대표체량야정정상관(r=0.923,P<0.001;r=0.972,P<0.001).결론 LOX화MMP-2재위암조직중적표체명현고우상응적암방위조직;재반유림파결전이적위암조직중,LOX화MMP-2표체고우무림파결전이적위암조직,차LOX화MMP-2표체정정상관,제시LOX화MMP-2대위암적발생화전이유촉진작용,차량자가능유협동작용.
Objective To compare the expressions of lysyl oxidase (LOX) and matrix metalloproteinases-2 (MMP-2) in gastric cancer and pericancerous tissues, in gastric cancers.with and without lymph node metastasis, and to analyze the effects of LOX and MMP-2 on tumor invasion and metastasis.Methods Gastric cancer and pericancerous tissues were collected from 46 patients who underwent surgery.Levels of LOX and MMP-2 mRNA were detected by RT-PCR.Protein abundance of LOX and MMP-2 was examined using Western blot.Results Expressions of LOX and MMP-2 mRNA, and protein in 46 gastric cancers were significantly higher than that in 46 pericancerous tissues.In gastric cancer with lymph node metastasis, the levels of LOX and MMP-2 mRNA and protein were higher than those in gastric cancers without lymph node metastasis ( P < 0.05 ).In the groups of gastric cancer with lymph node metastasis, expression of LOX was positively correlated with MMP-2 protein expression ( P < 0.01 ).Conclusions Expressions of LOX and MMP-2 in gastric cancer tissues are significantly higher than that in pericancerous tissues.The expressions of LOX and MMP-2 in gastric cancer with lymph node metastasis are higher than that in gastric cancer without lymph node metastasis.Expressions of LOX and MMP-2 are positively correlated.The results suggest that LOX and MMP-2 may promote the growth and metastasis of gastric cancer.
