CAJ | 학술논문

目的研究治疗白血病的有效药物。方法将槲皮素作用于培养的NB4细胞株，应用RT-PCR及Westem bolt方法，研究不同浓度槲皮素(30、60、90μmol/L)诱导细胞24、48、72、96、120h后，急性早幼粒细胞白血病NB4细胞株中突变型p53基因及蛋白表达的情况。结果证实其在30～90μmol/L浓度时对突变型p53基因无影响，而对突变型p53蛋白的表达有抑制作用。结论本研究为槲皮素应用于急性早幼粒细胞白血病的研究提供了有效手段。
목적연구치료백혈병적유효약물。방법장곡피소작용우배양적NB4세포주，응용RT-PCR급Westem bolt방법，연구불동농도곡피소(30、60、90μmol/L)유도세포24、48、72、96、120h후，급성조유립세포백혈병NB4세포주중돌변형p53기인급단백표체적정황。결과증실기재30～90μmol/L농도시대돌변형p53기인무영향，이대돌변형p53단백적표체유억제작용。결론본연구위곡피소응용우급성조유립세포백혈병적연구제공료유효수단。
Objective In order to study the value of drug treatment for leukemia, quercetin was delivered to the cultured NB4 cell line. Methods Using RT- PCR and western blot, we studied the expression of mutant p53 gene and protein after inducing 24, 48, 72, 96 and 120h by the quercetin with different concentrations (30, 60, 90μmol/L). Results We verified the results that quercetin with concentration of 30 ～90μmol/L could successfully inhibit the expression of mutant p53 protein, but the mutant p53 gene did not have. Conclusion That data suggested that quercetin would be an effective method for the therapy of leukemia.