CAJ | 학술논문

采用时间分辨荧光光谱测试系统,研究了超高产杂交水稻(Oryza sativa L.)两优培九(P9)和对照汕优63(SH 63)类囊体膜的荧光光谱特性和时间特性.以脉宽为120 ps,重复率为4MHz,波长为514 nm的Ar+激光分别激发P9和SH 63水稻类囊体膜荧光.通过对其超快荧光的时间特性和光谱特性比较研究发现:无论是在灌浆期还是在扬花期,P9水稻类囊体膜中光系统I激发能传递的速度比光系统Ⅱ的快;P9和SH 63两种水稻类囊体膜在灌浆期的激发能传递速度都比扬花期的快;两种水稻类囊体膜的光谱特性还给出,在从扬花期到灌浆期这一生长发育过程中,SH 63水稻类囊体膜的色素成分和结合状态发生了变化,而P9却没有出现这种变化.
채용시간분변형광광보측시계통,연구료초고산잡교수도(Oryza sativa L.)량우배구(P9)화대조산우63(SH 63)류낭체막적형광광보특성화시간특성.이맥관위120 ps,중복솔위4MHz,파장위514 nm적Ar+격광분별격발P9화SH 63수도류낭체막형광.통과대기초쾌형광적시간특성화광보특성비교연구발현:무론시재관장기환시재양화기,P9수도류낭체막중광계통I격발능전체적속도비광계통Ⅱ적쾌;P9화SH 63량충수도류낭체막재관장기적격발능전체속도도비양화기적쾌;량충수도류낭체막적광보특성환급출,재종양화기도관장기저일생장발육과정중,SH 63수도류낭체막적색소성분화결합상태발생료변화,이P9각몰유출현저충변화.
Thylakoid membrane preparations of super high-yield hybrid rice (Oryza sativa L.), Liangyoupeijiu(P9) and Shanyou 63 (SH 63) were used for investigating its spectral and time properties by using picosec-ond time-resolved fluorescence spectrum measuring system. The thylakoid membrane preparations of P9and SH 63 were excited by an Ar* laser with a pulse width of 120 ps, repetition rate of 4 MHz and wavelengthof 514 nm. The time constants of the excited energy transfer in these two varieties at flowering stage andgrain filling stage were calculated from the experimental data. Based on the comparative studies of thetime and spectral properties of the excited fluorescence in these ultrafast dynamic experiments thefollowing was found: at both the flowering stage and grain filling stage, the speed of the excitation energytransfer, in photosystem Ⅰ was faster than that in photosystem Ⅱ in P9 variety; and the speed of theexcitation energytransfer at grain filling stage was faster than those at flowering stage for both ricevarieties; the experiments also implied that the components and assembly of pigments in SH 63, but not inP9, changed during the process from flowering stage to grain filling stage for in these two rice varieties.