CAJ | 학술논문

背景:基质金属蛋白酶-7在肿瘤浸润转移中具有重要作用,并与多种肿瘤的预后有关.目的:通过检测喉鳞癌组织中基质金属蛋白酶-7的表达,探讨其在喉癌浸润转移中的作用及其与预后的关系.设计:以细胞为研究对象的开放性实验研究.单位:两所大学医院的耳鼻咽喉科和一所市级医院的耳鼻咽喉科.材料:实验于2002-04/2003-01在哈尔滨医科大学附属第二医院中心实验室完成,材料为喉鳞癌和癌旁非癌组织,基质金属蛋白酶-7多克隆抗体及免疫组化试剂盒.方法:采用免疫组化方法检测70例喉鳞癌和癌旁非癌组织蜡块标本中基质金属蛋白酶-7蛋白的表达,运用反转录-聚合酶链反应和Western-blot技术检测35例喉鳞癌和癌旁非癌组织冻存标本中基质金属蛋白酶-7的表达.主要结局观察:观察基质金属蛋白酶-7的mRNA和蛋白表达结果与喉鳞癌浸润、转移及预后的关系.结果:70例喉鳞癌组织中基质金属蛋白酶-7蛋白表达阳性率为77.1%(54/70),癌旁非癌组织为5.7%(4/70)(P＜0.01).35例冻存癌组织中基质金属蛋白酶-7 mRNA的表达阳性率为74.3%(24/35),癌旁非癌组织为5.7%(2/35)(P＜0.01).基质金属蛋白酶-7蛋白和mRNA水平的表达在浸润深度为T3～T4组明显高于T1～T2组,并且与颈淋巴结转移呈明显的正相关(P＜0.01).Western-blot显示28 ku(基质金属蛋白酶-7酶原)和19 ku(活化型MMP-7)条带吸光度值与浸润深度和淋巴结转移密切相关.Kaplan-Meier生存曲线显示基质金属蛋白酶-7蛋白表达强阳性组预后较弱阳性组及阴性组恶劣(Log-rank=4.755 9,8.951 3;P=0.029 2,0.002 8).结论:基质金属蛋白酶-7与喉鳞癌的浸润、转移及预后密切相关,可作为判断喉鳞癌浸润转移潜能及预后的生物学指标.
배경:기질금속단백매-7재종류침윤전이중구유중요작용,병여다충종류적예후유관.목적:통과검측후린암조직중기질금속단백매-7적표체,탐토기재후암침윤전이중적작용급기여예후적관계.설계:이세포위연구대상적개방성실험연구.단위:량소대학의원적이비인후과화일소시급의원적이비인후과.재료:실험우2002-04/2003-01재합이빈의과대학부속제이의원중심실험실완성,재료위후린암화암방비암조직,기질금속단백매-7다극륭항체급면역조화시제합.방법:채용면역조화방법검측70례후린암화암방비암조직사괴표본중기질금속단백매-7단백적표체,운용반전록-취합매련반응화Western-blot기술검측35례후린암화암방비암조직동존표본중기질금속단백매-7적표체.주요결국관찰:관찰기질금속단백매-7적mRNA화단백표체결과여후린암침윤、전이급예후적관계.결과:70례후린암조직중기질금속단백매-7단백표체양성솔위77.1%(54/70),암방비암조직위5.7%(4/70)(P＜0.01).35례동존암조직중기질금속단백매-7 mRNA적표체양성솔위74.3%(24/35),암방비암조직위5.7%(2/35)(P＜0.01).기질금속단백매-7단백화mRNA수평적표체재침윤심도위T3～T4조명현고우T1～T2조,병차여경림파결전이정명현적정상관(P＜0.01).Western-blot현시28 ku(기질금속단백매-7매원)화19 ku(활화형MMP-7)조대흡광도치여침윤심도화림파결전이밀절상관.Kaplan-Meier생존곡선현시기질금속단백매-7단백표체강양성조예후교약양성조급음성조악렬(Log-rank=4.755 9,8.951 3;P=0.029 2,0.002 8).결론:기질금속단백매-7여후린암적침윤、전이급예후밀절상관,가작위판단후린암침윤전이잠능급예후적생물학지표.
BACKGROUND: Matrix metalloproteinases-7 (MMP-7) plays an important role in the invasion and metastasis of cancer and is related to prognosis of many carcinomas.OBJECTIVE: To investigate the significance of MMP-7 expression in the invasion and metastasis of laryngeal cancer and explore the relationship between MMP-7 expression and the prognosis of laryngeal squamous cell cancer (LSCC).DEGIGN: An open experimental study based on the cells.SETTING: Two otolaryngology departments of two university hospitals and one otolaryngology department of a municipal hospital.MATERIALS: The experiment was conducted in the Center Laboratory of Second Affiliated Hospital of Harbin Medical University from April 2002 to January 2003. The materials were laryngeal cancer and corresponding neighboring noncancerous tissues, MMP-7 antibody and immunohistochemical kit.METHODS: The expression of MMP-7 protein in 70 samples of paraffin-embedded LSCC and corresponding neighboring noncancerous tissues was detected with immunohistochemical technique. Reverse transcription-polymerase chain reaction(RT-PCR) and Western blot technique were used to examine the expression of MMP-7 in 35 frozen specimens of LSCC and corresponding neighboring noncancerous tissues.MAIN OUTCOME MEASURES: The relationship of MMP-7 mRNA expression with the invasion depth(T grade), neck nodal metastasis and prognosis of LSCC.RESULTS: Immunohistochemical staining of MMP-7 was observed in 77.1% (54/70) of cancer tissues and in 5.7% (4/70) of neighboring noncancerous tissues( P ＜ 0.01) . The mRNA expression of MMP-7 was detected in 74.3% (24/35) of cancer tissues and in 5.7% (2/35) of neighboring noncancerous tissues( P ＜ 0.01 ). The expression of MMP-7 was higher in T3-T4 group than that in T1-T2 group(P ＜ 0.01), and was positively correlated with nodal metastasis( P ＜ 0. 01 ). Western blot showed that both 28 ku (latent form of MMP-7) and 19 ku(active form of MMP-7)bands had close relationship with T grade and neck nodal metastasis. Kaplan-Meier survival curve showed that the group with high MMP-7protein expression had poorer prognosis than the group with weak or negative MMP-7 protein expression(Log-rank = 4. 755 9, 8. 951 3; P = 0. 029 2,0.002 8).CONCLUSION: MMP-7 is closely correlated with the invasion, metastasis,and prognosis of LSCC, and it may serve as a marker in estimating the invasive and metastatic potency and prognosis of LSCC.