CAJ | 학술논문

海南岛的4个保护区和2个非保护区内普通野生稻与2种栽培稻(Nipponbare,9311)的Adh2基因序列在编码区共有单现突变9个,信息位点4个,同义突变8个,替代突变5个.内含子内有单现突变21个,信息位点29个,插入/缺失位点161个.Adh2基因在整个区域的多态性(π_R,θ_R)均为0.011,内含子区域的多态性明显高于编码区域.在编码区域,同义位点多态性明显高于替代位点.中性测试表明虽然Adh2各区域的净化选择在统计上不具有显著性,但编码区的净化选择作用明显大于内含子区域.聚类分析表明海南普通野生稻可以划分为2大类,其中一类(QH,LD)与中国大陆普通野生稻和栽培稻更接近,另一类(WC,WA,WN,DZ)则更接近东南亚普通野生稻.
해남도적4개보호구화2개비보호구내보통야생도여2충재배도(Nipponbare,9311)적Adh2기인서렬재편마구공유단현돌변9개,신식위점4개,동의돌변8개,체대돌변5개.내함자내유단현돌변21개,신식위점29개,삽입/결실위점161개.Adh2기인재정개구역적다태성(π_R,θ_R)균위0.011,내함자구역적다태성명현고우편마구역.재편마구역,동의위점다태성명현고우체대위점.중성측시표명수연Adh2각구역적정화선택재통계상불구유현저성,단편마구적정화선택작용명현대우내함자구역.취류분석표명해남보통야생도가이화분위2대류,기중일류(QH,LD)여중국대륙보통야생도화재배도경접근,령일류(WC,WA,WN,DZ)칙경접근동남아보통야생도.
The sequence polymorphism of Adh2 in six populations of Oryza rufipogon (WN, WA, WC, DZ, QH, and LD) from Haman and two Oryza sativa (Nipponbare, 9311) was analyzed. The result showed that there were nine singleton variable sites, four parsimony informative sites, eight synonymous sites and five replacement sites at coding region. There were 21 singleton variable sites, 29 parsimony informative sites and 161 indels sites at intron region. Adh2 polymorphism (π_R, θ_R) at entire region was 0.011. The polymorphism at intron was higher than that at coding region. At coding region, the polymorphism of synonymous sites was higher than that at replacement sites. Neutrality tests showed that there was more purified selection at coding region than at intron region, although no significant difference was found. Cluster analysis showed O. rufipogon from Hainan could be di-vided into two groups. One group (QH, LD) was clustered with O. rufipogon from China mainland and O. sativa. The other group was clustered with some O. rufipogon from southeast Asia.