CAJ | 학술논문

目的探讨脑源性神经营养因子(brain derived neurotrophic factor,BDNF)前体(proBDNF)在脊髓损伤中的作用.方法建立SD大鼠T10脊髓全横断损伤模型.以随机数字表法将动物分为抗体封闭组和人工脑脊液对照组.抗体封闭组应用椎管内释放proBDNF抗体以封闭脊髓内proBDNF,采用免疫组化评价proBDNF抗体封闭的效果,行为学BBB评分研究proBDNF鞘内封闭对脊髓全横断大鼠运动功能的影响.结果在全横断大鼠椎管内注入脑脊液的对照组没有观察到明显的运动功能改进,而proBDNF抗体鞘内封闭不仅有效减少脊髓内proBDNF,而且明显改善大鼠行为功能.proBDNF抗体封闭组行为学BBB评分较人工脑脊液对照组明显升高.结论通过proBDNF抗体鞘内封闭减少脊髓内proBDNF能有效促进全横断脊髓损伤大鼠行为功能部分恢复.
목적 탐토뇌원성신경영양인자(brain derived neurotrophic factor,BDNF)전체(proBDNF)재척수손상중적작용.방법 건립SD대서T10척수전횡단손상모형.이수궤수자표법장동물분위항체봉폐조화인공뇌척액대조조.항체봉폐조응용추관내석방proBDNF항체이봉폐척수내proBDNF,채용면역조화평개proBDNF항체봉폐적효과,행위학BBB평분연구proBDNF초내봉폐대척수전횡단대서운동공능적영향.결과 재전횡단대서추관내주입뇌척액적대조조몰유관찰도명현적운동공능개진,이proBDNF항체초내봉폐불부유효감소척수내proBDNF,이차명현개선대서행위공능.proBDNF항체봉폐조행위학BBB평분교인공뇌척액대조조명현승고.결론 통과proBDNF항체초내봉폐감소척수내proBDNF능유효촉진전횡단척수손상대서행위공능부분회복.
Objective To investigate the role of precursor of brain derived neurotrophic factor (proBDNF) in the improvement of motor function of rats subjected to cord transection. Methods Spinal cord transection was performed at T10 level. The rats were divided into sham operation group and artificial cerebrospinal fluid group. Then, the rats were subjected to proBDNF antibody treatment. The efficiency of proBDNF block was also determined by immunohistochemistry. BBB scale scores were used to e-valuate the effect of ProBDNF block on the motor function of the rats with spinal cord transection. Results Compared with control rats, proBDNF treatment showed a marked down-regulation of proBDNF protein in the spinal cord and could significantly improve the motor function. BBB scores in proBDNF block group was higher than those in artificial cerebrospinal fluid group. Conclusion The proBDNF block is available to the recovery of the motor function in hindlimbs in cord transected rats, suggesting that proBDNF could be as a member of candidate molecules for SCI therapy in the future.