中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2011年
1期
2-6
,共5页
百草枯%细胞毒性试验%PC12细胞%基因表达
百草枯%細胞毒性試驗%PC12細胞%基因錶達
백초고%세포독성시험%PC12세포%기인표체
Paraquat%Cytotoxicity tests%PC 12 cells%Gene expression
目的 探讨百草枯(PQ)对大鼠肾上腺嗜铬细胞瘤(PC12)细胞毒性作用和对miR-133b表达的影响.方法 以50、100、300 μmol/L PQ分别处理PC12细胞24 h,用噻唑蓝(MTT)法检测PC12细胞毒性;以100、300 μmol/LPQ分别处理PC12细胞24 h,用Annexin V-FITC/PI法流式细胞仪检测细胞凋亡,用荧光定量反转录聚合酶链反应(RT-PCR)法检测miR-133b相对表达水平.结果 与对照组比较,100、300 μmol/L PQ处理细胞24 h后细胞存活率明显下降,差异有统计学意义(P＜0.05或P＜0.01).与对照组比较,100、300μmol/L PQ处理细胞24 h后细胞凋亡率明显升高,差异有统计学意义(P＜0.01).300 μmol/LPQ处理细胞24 h可诱导细胞miR-133b表达,差异有统计学意义(P＜0.05).结论 PQ致PC12细胞损害和细胞凋亡时miR-133b的表达上调.
目的 探討百草枯(PQ)對大鼠腎上腺嗜鉻細胞瘤(PC12)細胞毒性作用和對miR-133b錶達的影響.方法 以50、100、300 μmol/L PQ分彆處理PC12細胞24 h,用噻唑藍(MTT)法檢測PC12細胞毒性;以100、300 μmol/LPQ分彆處理PC12細胞24 h,用Annexin V-FITC/PI法流式細胞儀檢測細胞凋亡,用熒光定量反轉錄聚閤酶鏈反應(RT-PCR)法檢測miR-133b相對錶達水平.結果 與對照組比較,100、300 μmol/L PQ處理細胞24 h後細胞存活率明顯下降,差異有統計學意義(P＜0.05或P＜0.01).與對照組比較,100、300μmol/L PQ處理細胞24 h後細胞凋亡率明顯升高,差異有統計學意義(P＜0.01).300 μmol/LPQ處理細胞24 h可誘導細胞miR-133b錶達,差異有統計學意義(P＜0.05).結論 PQ緻PC12細胞損害和細胞凋亡時miR-133b的錶達上調.
목적 탐토백초고(PQ)대대서신상선기락세포류(PC12)세포독성작용화대miR-133b표체적영향.방법 이50、100、300 μmol/L PQ분별처리PC12세포24 h,용새서람(MTT)법검측PC12세포독성;이100、300 μmol/LPQ분별처리PC12세포24 h,용Annexin V-FITC/PI법류식세포의검측세포조망,용형광정량반전록취합매련반응(RT-PCR)법검측miR-133b상대표체수평.결과 여대조조비교,100、300 μmol/L PQ처리세포24 h후세포존활솔명현하강,차이유통계학의의(P＜0.05혹P＜0.01).여대조조비교,100、300μmol/L PQ처리세포24 h후세포조망솔명현승고,차이유통계학의의(P＜0.01).300 μmol/LPQ처리세포24 h가유도세포miR-133b표체,차이유통계학의의(P＜0.05).결론 PQ치PC12세포손해화세포조망시miR-133b적표체상조.
Objective To investigate the effect of paraquat on induction of cell damage and miR-133b expression in PC 12 cells. Methods Cytoyoxicity of PC 12 cells was measured by MTT assay, following the PC 12 cells treatment with 50,100, or 300μmol/L paraquat. Cell apoptosis was examined by the method of Annexin V-FITC/P1 in flow cytometry (FCM) and the relative level of miR-133b expression was measured by real time RT-PCR, following the PC12 cells treatment with 100 or 300 μmol/L paraquat. Results Survival rate of PC 12 cells treated with 100 or 300 μmol/L paraquat was lower than that of the vehicle control group (P＜0.01,P＜0.05), in the dose dependent pattern. Apoptotic rate of PC 12 cells treated with 100, 300 μmol/L paraquat was higher than that of the vehicle control group (P＜0.05). The relative level of miR-133b expression of PC 12 cells treated with 300 μmol/L paraquat was higher than that of the vehicle control group (P＜0.05). Conclusions Paraquat may cause cell damage and induce apoptosis in PC 12 cells, and induce miR- 133b expression.
