中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2011年
1期
32-36
,共5页
詹鹤琴%朱雄增%李小秋%周晓燕
詹鶴琴%硃雄增%李小鞦%週曉燕
첨학금%주웅증%리소추%주효연
淋巴瘤,T细胞,外周%淋巴瘤,滤泡型%免疫表型分型%基因重排
淋巴瘤,T細胞,外週%淋巴瘤,濾泡型%免疫錶型分型%基因重排
림파류,T세포,외주%림파류,려포형%면역표형분형%기인중배
Lymphoma,T cell,peripheral%Lymphoma,follicular%Immunophenotyping%Gene rearrangement
目的 观察滤泡变型外周T细胞淋巴瘤的临床病理特征和遗传学改变,探讨其与血管免疫母细胞性T细胞淋巴瘤的关系.方法 对2例滤泡变型外周T细胞淋巴瘤进行临床资料分析和组织病理学观察,以免疫组织化学染色(除CXCL13为三步SP法外,其余均为EnVision法)和EBER原位杂交方法分析肿瘤免疫表型和EB病毒感染状况,并分别以聚合酶链反应(PCR)和逆转录聚合酶链反应(RT-PCR)方法检测IgH、T细胞受体(TCR)基因克隆性重排和ITK/SYK融合基因转录本表达情况.结果 2例患者均以浅表淋巴结肿大为主要表现.镜下观察,例1示紧密排列的滤泡/结节状淋巴组织增生,结节内无明显生发中心,肿瘤细胞以中、小透亮细胞为主.例2左颈病变也主要表现为滤泡/结节状淋巴组织增生,但右颈病变大部分区域呈现典型血管免疫母细胞性T细胞淋巴瘤组织学图像.2例肿瘤细胞均表达滤泡辅助T细胞标记(CD3、CD4、CD10、PD1及CXCL13阳性).例1的EBER原位杂交阴性.例2有散在EBER阳性的反应性转化B细胞存在.分子遗传学检测示2例均有TCR基因单克隆性重排而无ITK/SYK融合基因表达.结论滤泡变型外周T细胞淋巴瘤作为一种少见的外周T细胞淋巴瘤的形态学变型,不但免疫表型和血管免疫母细胞性T细胞淋巴瘤相似,其组织学形态也与后者有着相似或重叠之处,提示两者密切相关或存在互相演进关系.
目的 觀察濾泡變型外週T細胞淋巴瘤的臨床病理特徵和遺傳學改變,探討其與血管免疫母細胞性T細胞淋巴瘤的關繫.方法 對2例濾泡變型外週T細胞淋巴瘤進行臨床資料分析和組織病理學觀察,以免疫組織化學染色(除CXCL13為三步SP法外,其餘均為EnVision法)和EBER原位雜交方法分析腫瘤免疫錶型和EB病毒感染狀況,併分彆以聚閤酶鏈反應(PCR)和逆轉錄聚閤酶鏈反應(RT-PCR)方法檢測IgH、T細胞受體(TCR)基因剋隆性重排和ITK/SYK融閤基因轉錄本錶達情況.結果 2例患者均以淺錶淋巴結腫大為主要錶現.鏡下觀察,例1示緊密排列的濾泡/結節狀淋巴組織增生,結節內無明顯生髮中心,腫瘤細胞以中、小透亮細胞為主.例2左頸病變也主要錶現為濾泡/結節狀淋巴組織增生,但右頸病變大部分區域呈現典型血管免疫母細胞性T細胞淋巴瘤組織學圖像.2例腫瘤細胞均錶達濾泡輔助T細胞標記(CD3、CD4、CD10、PD1及CXCL13暘性).例1的EBER原位雜交陰性.例2有散在EBER暘性的反應性轉化B細胞存在.分子遺傳學檢測示2例均有TCR基因單剋隆性重排而無ITK/SYK融閤基因錶達.結論濾泡變型外週T細胞淋巴瘤作為一種少見的外週T細胞淋巴瘤的形態學變型,不但免疫錶型和血管免疫母細胞性T細胞淋巴瘤相似,其組織學形態也與後者有著相似或重疊之處,提示兩者密切相關或存在互相縯進關繫.
목적 관찰려포변형외주T세포림파류적림상병리특정화유전학개변,탐토기여혈관면역모세포성T세포림파류적관계.방법 대2례려포변형외주T세포림파류진행림상자료분석화조직병이학관찰,이면역조직화학염색(제CXCL13위삼보SP법외,기여균위EnVision법)화EBER원위잡교방법분석종류면역표형화EB병독감염상황,병분별이취합매련반응(PCR)화역전록취합매련반응(RT-PCR)방법검측IgH、T세포수체(TCR)기인극륭성중배화ITK/SYK융합기인전록본표체정황.결과 2례환자균이천표림파결종대위주요표현.경하관찰,례1시긴밀배렬적려포/결절상림파조직증생,결절내무명현생발중심,종류세포이중、소투량세포위주.례2좌경병변야주요표현위려포/결절상림파조직증생,단우경병변대부분구역정현전형혈관면역모세포성T세포림파류조직학도상.2례종류세포균표체려포보조T세포표기(CD3、CD4、CD10、PD1급CXCL13양성).례1적EBER원위잡교음성.례2유산재EBER양성적반응성전화B세포존재.분자유전학검측시2례균유TCR기인단극륭성중배이무ITK/SYK융합기인표체.결론려포변형외주T세포림파류작위일충소견적외주T세포림파류적형태학변형,불단면역표형화혈관면역모세포성T세포림파류상사,기조직학형태야여후자유착상사혹중첩지처,제시량자밀절상관혹존재호상연진관계.
Objective To observe the clinicopathologic and genetic features of follicular variant of peripheral T-cell lymphoma (FV-PTCL), with particular attention to the relationship of this type of lymphoma with angioimmunoblastic T-cell lymphoma (AIIL). Methods The clinical data, hemstoxylin and eosin-stained sections of lymph node biopsies from 2 FV-PTCL cases were reviewed.Immunohistochemical phenotyping and detection of EBV-encoded RNAs (EBER) through in situ hybridization (ISH) were performed. The EnVision two-step method was used for all antibodies except CXCL13 (by using three-step streptavidin immunoperoxidase method). Analysis of clonality and ITK/SYK gene rearrangement was conducted using PCR and RT-PCR assays, respectively. Results Clinically, the two patients presented with superficial lymphadenopathy similarly. Histologically, case 1 showed a follicular/nodular lymphoid proliferation without marked germinal centers. The neoplastic cells comprised mainly medium sized cells with abundant, sometimes clear cytoplasms. Similar histologic findings were seen in case 2 in addition to a concurrent component mimicking typical AITL noticed. Of both cases, the neoplastic cells showed positive reactivity to CD3, CD4, CD10, PD1, and CXCL13. Positive hybridization signals for EBER were only seen in case 2, and double stains demonstrated that those EBV-positive cells were mostly the reactive transformed B-cells. Monoclonal T-cell proliferation was proved by the rearranged TCR gene detection in both cases. Neither of the current cases expressed ITK/SYK fusion transcripts. Conclusion FV-PTCL shows the similar or overlapped morphological and immunophenotypic features to those of AITL,possibly suggesting the presence of a potential relationship between these two types of lymphomas.