CAJ | 학술논문

目的观察白细胞介素-17(IL-17)对HaCaT细胞分泌血管内皮生长因子(VEGF)的影响及紫草素的干预作用。方法收集IL-17刺激组和紫草素处理后HaCaT细胞及其培养土清，分别用双抗体夹心酶联免疫吸附法(ELISA)及实时荧光免疫定量-聚合酶链反应法(RT-PCR)检测VEGF含量。应用细胞计数盒-8(CCK-8)检测各组HaCaT细胞活力。结果与对照组相比，不同时间IL-17刺激组HaCaT细胞及其培养上清中VEGF表达均高于对照组(P＜0.001)；紫草素处理组HaCaT细胞及其培养上清VEGF表达均低于IL-17处理组(P＜0.001)；与对照组比较，CCK-8检测各组HaCaT细胞活力无明显差异(P＞0.05)。结论 IL-17可促进HaCaT细胞分泌VEGF，呈时间依赖型，而紫草素对其具有抑制作用。
목적 관찰백세포개소-17(IL-17)대HaCaT세포분비혈관내피생장인자(VEGF)적영향급자초소적간예작용。방법 수집IL-17자격조화자초소처리후HaCaT세포급기배양토청，분별용쌍항체협심매련면역흡부법(ELISA)급실시형광면역정량-취합매련반응법(RT-PCR)검측VEGF함량。응용세포계수합-8(CCK-8)검측각조HaCaT세포활력。결과 여대조조상비，불동시간IL-17자격조HaCaT세포급기배양상청중VEGF표체균고우대조조(P＜0.001)；자초소처리조HaCaT세포급기배양상청VEGF표체균저우IL-17처리조(P＜0.001)；여대조조비교，CCK-8검측각조HaCaT세포활력무명현차이(P＞0.05)。결론 IL-17가촉진HaCaT세포분비VEGF，정시간의뢰형，이자초소대기구유억제작용。
Objective To investigate whether IL-17 could stimulate the vascular endothelial growth factor (VEGF) production on HaCaT cells alone. We also investigated whether shikonin could inhibited the proinflamation effects of interleukin-17(IL-17) acting on HaCaT cells. Methods We examined the expression of VEGF by double antibody sandwich enzyme-linked immunosorbent assay ( ELISA ) and realtime polymerase chain reaction(RT-PCR) in HaCaT cells and the cell supernatant. The viability of HaCaT cells in the drug group was detected by the Cell Counting Kit-8 (CCK-8). Results The expression of VEGF in different time IL-17-stimulated groups on HaCaT cells and the cell supernatant were higher than the control group( P＜0.001 ). The expression of VEGF in different drug treatment groups on HaCaT cells and the cell supematant were lower than the stimulated group by IL-17 ( P＜0. 001 ). The cell viability of different drug treatment groups have no significant difference( P＞0.05 ). Conclusion We show that IL-17 specifically and time-dependently augmented and induced VEGF expression on HaCaT cells and the cell supernatant Then shikonin markedly inhibited the increase tengency of IL-17 effection on HaCaT cells and the cell supematant level.