CAJ | 학술논문

目的探讨Pokemon基因与顺铂作用机制的相关性.方法体外培养人肺腺癌细胞株(A549、AGZY83-a),人肺鳞癌细胞株(HE-99),人肺巨细胞癌细胞株(95D)等4种肺癌细胞株,实验组在培养过程中加入顺铂,对照组在培养过程不加入顺铂;应用RT-PCR、Western印迹检测两组Pokemon基因mRNA和蛋白表达的情况.结果 4种细胞株中Pokemon mRNA及蛋白均呈较高表达.实验组中细胞株经顺铂作用后Pokemon基因表达没有明显变化,与对照组相比差异无统计学意义(均P>0.05).结论顺铂对A549、AGZY83-a、HE-99、95D细胞株中Pokemon基因的表达没有明显影响.
목적 탐토Pokemon기인여순박작용궤제적상관성.방법 체외배양인폐선암세포주(A549、AGZY83-a),인폐린암세포주(HE-99),인폐거세포암세포주(95D)등4충폐암세포주,실험조재배양과정중가입순박,대조조재배양과정불가입순박;응용RT-PCR、Western인적검측량조Pokemon기인mRNA화단백표체적정황.결과 4충세포주중Pokemon mRNA급단백균정교고표체.실험조중세포주경순박작용후Pokemon기인표체몰유명현변화,여대조조상비차이무통계학의의(균P>0.05).결론 순박대A549、AGZY83-a、HE-99、95D세포주중Pokemon기인적표체몰유명현영향.
Objective To investigate the correlation between Pokemon gene and cisplatin mechanism. Methods Human lung adenocarcinoma cells of the lines A549 and AGZY83-a, human lung squamous carcinoma cells of the line HE-99, and human giant cell lung cancer cells of the line 95D were cultured and cisplatin was added into the medium. Other lung cancer cells of the above mentioned lines were cultured in the medium without cisplatin and were used as control groups. RT-PCR and Western blotting were used to detect the mRNA and protein expression of Pokemon. Results Pokemon mRNA and protein were expressed highly in all the 4 cell lines. The Pokemon gene expression did not changed significantly after cisplatin treatment groups. There were not significant differences in the mRNA and protein expression of Pokemon among the 4 experiment groups and the control groups ( all P>0.05 ). Conclusion Cisplatin has no effect on the Pokemon gene expression of the human lung cancer cells.