中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2005年
31期
224-225
,共2页
衰老/中药疗法%睾丸%睾酮%增殖细胞核抗原
衰老/中藥療法%睪汍%睪酮%增殖細胞覈抗原
쇠로/중약요법%고환%고동%증식세포핵항원
背景:何首乌颗粒由何首乌、肉苁蓉、怀牛膝等组成,具有补肝肾、益精血、延年益寿的功效.目的:观察中药何首乌颗粒对D-半乳糖致亚急性衰老大鼠血清睾酮含量及睾丸生精细胞增殖细胞核抗原表达的影响.设计:完全随机分组设计,对照实验.单位:承德医学院基础医学研究所.材料:实验于2003-04/07在承德医学院基础医学研究所完成.选用成年雄性SD大鼠32只.大鼠被随机分为4组:正常对照组、模型组、用药组和阴性对照组,每组8只.方法:[1]模型组、用药组、阴性对照组大鼠均采用D-半乳糖连续腹腔注射200 mg/(kg·d),1次/d,共40 d,制作亚急性衰老的大鼠模型.模型组大鼠于造模成功后不再作任何处理;用药组大鼠于造模成功后每天用何首乌颗粒(由灸何首乌、怀牛膝、肉从蓉、茯苓、丹参、淫羊藿六味中药组成,经熬制后每毫升含生药1.5g)灌胃;阴性对照组大鼠在造模成功后每天灌胃同等剂量的生理盐水,时间同用药组大鼠.正常对照组大鼠不造成衰老模型,干预措施同阴性对照组.[2]血清睾酮水平酶联免疫吸附法检测.增殖细胞核抗原的表达情况采用免疫组化法检测,以细胞核呈桔黄色或棕黄色颗粒为阳性.[3]计量差异性测定采用t检验.主要观察指标:各组大鼠血清睾酮含量、睾丸生精细胞增殖细胞核抗原的表达比较.结果:大鼠32只均进入结果分析.[1]血清睾酮水平:模型组大鼠血清睾酮水平明显低于正常对照组[(0.52±0.14),(1.26±0.32)μg/L,P＜0.05];用药组明显高于模型组[(1.16±0.32)μg/L,P＜0.05].[2]大鼠睾丸增殖细胞核抗原的阳性细胞率:模型组明显低于正常对照组[(23.26±3.38)%,(39.15±3.48)%,P＜0.05];用药组大鼠明显高于模型组[(37.47±3.18)%,P＜0.05].[3]增殖细胞核抗原在生精小管中的分布及变化:各组切片均可见增殖细胞核抗原阳性染色,免疫阳性产物位于细胞核,通常呈棕黄色颗粒,小部分呈弥散状;免疫阳性细胞主要是精原细胞,另外还有少量初级精母细胞、支持细胞和肌样细胞核着色.结论:何首乌颗粒可以逆转衰老大鼠血清睾酮含量及睾丸生精细胞增殖细胞核抗原表达水平的下降,从而延缓生殖功能的退化,具有一定延缓衰老的作用.
揹景:何首烏顆粒由何首烏、肉蓯蓉、懷牛膝等組成,具有補肝腎、益精血、延年益壽的功效.目的:觀察中藥何首烏顆粒對D-半乳糖緻亞急性衰老大鼠血清睪酮含量及睪汍生精細胞增殖細胞覈抗原錶達的影響.設計:完全隨機分組設計,對照實驗.單位:承德醫學院基礎醫學研究所.材料:實驗于2003-04/07在承德醫學院基礎醫學研究所完成.選用成年雄性SD大鼠32隻.大鼠被隨機分為4組:正常對照組、模型組、用藥組和陰性對照組,每組8隻.方法:[1]模型組、用藥組、陰性對照組大鼠均採用D-半乳糖連續腹腔註射200 mg/(kg·d),1次/d,共40 d,製作亞急性衰老的大鼠模型.模型組大鼠于造模成功後不再作任何處理;用藥組大鼠于造模成功後每天用何首烏顆粒(由灸何首烏、懷牛膝、肉從蓉、茯苓、丹參、淫羊藿六味中藥組成,經熬製後每毫升含生藥1.5g)灌胃;陰性對照組大鼠在造模成功後每天灌胃同等劑量的生理鹽水,時間同用藥組大鼠.正常對照組大鼠不造成衰老模型,榦預措施同陰性對照組.[2]血清睪酮水平酶聯免疫吸附法檢測.增殖細胞覈抗原的錶達情況採用免疫組化法檢測,以細胞覈呈桔黃色或棕黃色顆粒為暘性.[3]計量差異性測定採用t檢驗.主要觀察指標:各組大鼠血清睪酮含量、睪汍生精細胞增殖細胞覈抗原的錶達比較.結果:大鼠32隻均進入結果分析.[1]血清睪酮水平:模型組大鼠血清睪酮水平明顯低于正常對照組[(0.52±0.14),(1.26±0.32)μg/L,P＜0.05];用藥組明顯高于模型組[(1.16±0.32)μg/L,P＜0.05].[2]大鼠睪汍增殖細胞覈抗原的暘性細胞率:模型組明顯低于正常對照組[(23.26±3.38)%,(39.15±3.48)%,P＜0.05];用藥組大鼠明顯高于模型組[(37.47±3.18)%,P＜0.05].[3]增殖細胞覈抗原在生精小管中的分佈及變化:各組切片均可見增殖細胞覈抗原暘性染色,免疫暘性產物位于細胞覈,通常呈棕黃色顆粒,小部分呈瀰散狀;免疫暘性細胞主要是精原細胞,另外還有少量初級精母細胞、支持細胞和肌樣細胞覈著色.結論:何首烏顆粒可以逆轉衰老大鼠血清睪酮含量及睪汍生精細胞增殖細胞覈抗原錶達水平的下降,從而延緩生殖功能的退化,具有一定延緩衰老的作用.
배경:하수오과립유하수오、육종용、부우슬등조성,구유보간신、익정혈、연년익수적공효.목적:관찰중약하수오과립대D-반유당치아급성쇠로대서혈청고동함량급고환생정세포증식세포핵항원표체적영향.설계:완전수궤분조설계,대조실험.단위:승덕의학원기출의학연구소.재료:실험우2003-04/07재승덕의학원기출의학연구소완성.선용성년웅성SD대서32지.대서피수궤분위4조:정상대조조、모형조、용약조화음성대조조,매조8지.방법:[1]모형조、용약조、음성대조조대서균채용D-반유당련속복강주사200 mg/(kg·d),1차/d,공40 d,제작아급성쇠로적대서모형.모형조대서우조모성공후불재작임하처리;용약조대서우조모성공후매천용하수오과립(유구하수오、부우슬、육종용、복령、단삼、음양곽륙미중약조성,경오제후매호승함생약1.5g)관위;음성대조조대서재조모성공후매천관위동등제량적생리염수,시간동용약조대서.정상대조조대서불조성쇠로모형,간예조시동음성대조조.[2]혈청고동수평매련면역흡부법검측.증식세포핵항원적표체정황채용면역조화법검측,이세포핵정길황색혹종황색과립위양성.[3]계량차이성측정채용t검험.주요관찰지표:각조대서혈청고동함량、고환생정세포증식세포핵항원적표체비교.결과:대서32지균진입결과분석.[1]혈청고동수평:모형조대서혈청고동수평명현저우정상대조조[(0.52±0.14),(1.26±0.32)μg/L,P＜0.05];용약조명현고우모형조[(1.16±0.32)μg/L,P＜0.05].[2]대서고환증식세포핵항원적양성세포솔:모형조명현저우정상대조조[(23.26±3.38)%,(39.15±3.48)%,P＜0.05];용약조대서명현고우모형조[(37.47±3.18)%,P＜0.05].[3]증식세포핵항원재생정소관중적분포급변화:각조절편균가견증식세포핵항원양성염색,면역양성산물위우세포핵,통상정종황색과립,소부분정미산상;면역양성세포주요시정원세포,령외환유소량초급정모세포、지지세포화기양세포핵착색.결론:하수오과립가이역전쇠로대서혈청고동함량급고환생정세포증식세포핵항원표체수평적하강,종이연완생식공능적퇴화,구유일정연완쇠로적작용.
BACKGROUND: Fleece Flower Root Granule, which consists of Fleece Flower Root, Desert-living Root, Achyranthes Root, and so on,possesses the effects of nourishing the liver and kidney, supplementing essence and blood, and prolonging life.OBJECTIVE: To investigate the effect of. Fleece Flower Root Granule, a Chinese remedy, on the content of serum testosterone and the expression of proliferating cell nuclear antigen of testicular spermatogenic cell in subacute aging rat induced by D-galactose.DESIGN: A controlled study with completely randomized grouping design.SETTING: Institute of Basic Medicine of Chengde Medical College.MATERIALS: The experiment was conducted in Institute of Basic Medicine of Chengde Medical College from April to July 2003. Totally 32 adult male SD rats were at random divided into 4 groups: normal control group, model group, drug group and negative control group,with 8 in each group.METHODS: [1] The rats in model group, drug group and negative control group were intraperitoneally injected D-galactose, 200 mg/kg ,once a day for consecutive 40 days to establish subacute aging rat models. After model was set up, no treatment was given further for rats in model group; while rats in drug group were intragastrically given Fleece Flower Root Granule (The granule was made from sex Chinese medicinal herbs of Fleece Flower Root, Achyranthes, Root Desertliving Root, Poria, Red Sage Root and Epimedium, the concentration was 1.5 g/L in raw drug after decoction and preparation); rats in negative group were intragastrically given normal saline, in the same volume as that of the Granule and the time as the same with that for the rats in drug group. Rats in normal control group, with no model establishment, were given the same intervention with that for rats in negative group. [2]Enzyme-linked immunosorbent assays (ELISA) was used for the level of serum testosterone, and inmmunohistochemistry was used for the condition of expression of proliferating cell nuclear antigen, with the karyon in orange brawn yellow being taken as positive. [3]Measurement difference was analyzed by t-test.MAIN OUTCOME MEASURES: The content of serum testosterone and the expression of proliferating cell nuclear antigen of testicular spermatogenic cell of rats in each group.RESULTS: All 32 rats entered the result analysis. [1] The level of serum testosterone:the level of testosterone in model group was obviously lower than that in normal control group [(0.52±0.14),(1.26±0.32)μg/L, P＜ 0.05]; the level in drug group was obviously higher than that in model group [(1.16±0.32)μg/L, P ＜ 0.05]. [2] The positive rate of testicular proliferating cell nuclear antigen in rats: The rate in model group was obviously lower than that in normal control group [(23.26±3.38)%,(39.15±3.48)%, P ＜ 0.05]; and the rate in drug group was obviously higher than that in model group [(37.47±3.18)%, P ＜ 0.05]. [3]rhe distribution and change of proliferating cell nuclear antigen in seminiferous tubule: The positive staining of proliferating cell nuclear antigen was found in slices of all groups, the immunological positive products were located at karyon, usually in brown yellow granules, only a small part of them in diffuse state; the immunological positive cells were mainly the spermospores, besides a small quantity were spermiocyte, supporting the cell and myoid cell nuclear to be stained.CONCLUSION: Fleece Flower Root Granule can increase the content of serum testosterone and reduce the level of expression of proliferating cell nuclear antigen of testicular spermatogenic cell in aging rat, thus delaying degeneration of genital function, possessing a certain role in delaying aging.
