CAJ | 학술논문

目的:观测通脉新复方对系膜增生性肾炎(MsPGN)病理学改变的影响.方法:采用抗胸腺抗血清(ATS)制备大鼠MsPGN肾炎模型及行疲劳游泳致气虚血瘀证模型,分别ig给药高、中、低剂量(1.2,0.6,0.3 g·kg-1)通脉新复方4周.4周后进行肾组织病理学检查、免疫荧光检测IgG以及免疫组化法测定肾小球内细胞核增殖抗原PCNA的表达.结果:通脉新复方能抑制肾小球系膜增生,降低系膜区基质占毛细血管丛面积的百分比,减少大鼠肾脏系膜区IgG沉积,显著降低肾小球内PCNA阳性细胞数.结论:通脉新复方能改善系膜增生性肾炎大鼠模型肾组织病理损伤.
목적:관측통맥신복방대계막증생성신염(MsPGN)병이학개변적영향.방법:채용항흉선항혈청(ATS)제비대서MsPGN신염모형급행피로유영치기허혈어증모형,분별ig급약고、중、저제량(1.2,0.6,0.3 g·kg-1)통맥신복방4주.4주후진행신조직병이학검사、면역형광검측IgG이급면역조화법측정신소구내세포핵증식항원PCNA적표체.결과:통맥신복방능억제신소구계막증생,강저계막구기질점모세혈관총면적적백분비,감소대서신장계막구IgG침적,현저강저신소구내PCNA양성세포수.결론:통맥신복방능개선계막증생성신염대서모형신조직병리손상.
Objective: To observe the therapeutic effects of effective compounds which were extracted from TongMai. Methods: SD rats experimential model with MsPGN were induced by subcutaneously injection rabbit anti-rat thymocyte serum(ATS) and forced swim. The rats were respectively ig with different dose(1.2,0.6,0.3 g'kg-1) of effective compound of TongMai for 4 weeks. Then the proliferative degree of mesangium was examined by HE stain, PAS stain and immunfluorescent respectively. Mesagium area proportion in glomerular capillaries area was calculated using image analyzing system. The expression of PCNA in the glomeruli was examined by immunohis to chemistry. Results: The effective compounds of TongMai can control the hyperplasia of the glomemlar mesangial cells (GMG)and the mainifold of the mesangial matrix, and prevent the deposit of IgG in mesangium. The proportions in glomerular capillaries area in model control group were significantly higher than that in the treatment groups; The express of proliferative cell nuclear antigen (PCNA) were ascended either. Conclusion: The effective compound of TongMai can improve the damage s of pathology in glomerular.