CAJ | 학술논문

目的:观察黄酮对氧化剂所诱导的视网膜色素上皮细胞(RPE)的保护作用.方法:在体研究中,预先给予5g/L黄酮滴眼液(3次/d),1wk后舌下静脉注射NaIO3诱导大鼠RPE变性,在2和4wk末,采用视网膜电图(ERG)测量C波.离体研究中,采用缺氧、H2O2、NaN3和t-BHP诱导RPE细胞损伤,并用MTT法检测细胞的存活率.结果:ERG的C波结果表明,第4wk末,黄酮抑制了由NaIO3诱导的大鼠RPE变性.离体研究结果表明,黄酮对多种氧化剂所诱导的RPE细胞损伤具有保护作用.结论:黄酮对氧化诱导的在体和离体视网膜色素上皮细胞均具有保护作用.
목적:관찰황동대양화제소유도적시망막색소상피세포(RPE)적보호작용.방법:재체연구중,예선급여5g/L황동적안액(3차/d),1wk후설하정맥주사NaIO3유도대서RPE변성,재2화4wk말,채용시망막전도(ERG)측량C파.리체연구중,채용결양、H2O2、NaN3화t-BHP유도RPE세포손상,병용MTT법검측세포적존활솔.결과:ERG적C파결과표명,제4wk말,황동억제료유NaIO3유도적대서RPE변성.리체연구결과표명,황동대다충양화제소유도적RPE세포손상구유보호작용.결론:황동대양화유도적재체화리체시망막색소상피세포균구유보호작용.
AIM: To investigate the effect of flavone on oxidation-induced injury in retinal pigment epithelium(RPE) cells. METHODS: In in vivo studies, NaIO3-induced RPE degeneration in rat eyes were treated with 5g/L flavone eye drops 3 times a day for 1 week before and 4 weeks after NaIO3injection. At the end of 2 and 4 weeks, all rats were measured c-wave by electroretinogram(ERG). In in vitro studies,ARPE-19 cells were treated with hypoxia, H2O2, NaN3and t-BHP to induce cell damages. MTT assay was used to measure the viable cells. RESULTS: The ERG c-wave results showed that flavone reversed NaIO3-induced injury at the end of 4 weeks. In vitro results showed flavone reversed the various oxidants-induced injuries in RPE cells.CONCLUSION: Flavone could prevent the RPE from oxidation-induced injury both in vivo and in vitro.