CAJ | 학술논문

本文尝试将中红外光谱(MIR)应用于肉苁蓉药材的质量评价和分析中.首先比较肉苁蓉药材粉末样品及其3种主要活性成分提取物,包括本乙醇总苷(PeGs)、甘露醇(Man)和多糖的红外光谱图差异,得出3种提取物的特征红外吸收峰,确定药材样品各特征峰的强弱与PeGs和Man含量高低的关系.然后用Spectrum v5.02软件计算得出不同波段内药材图谱与两种提取物图谱的红外相似系数,通过SPSS11.0软件分析发现,药材PeGs的含量(y)和1800~1200cm 1范围内的红外相似系数(x)呈极显著相关(R2=0.9927),回归方程:y=19.846x+0.0623.同样,药材Man的含量(y)与1100～600cm-1波数范围的红外相似系数(x)呈极显著相关(R2=0.9705),回归方程:y=13.855x- 0.1714.因此,MIR可作为肉苁蓉药材质量定性和半定量分析的快速有效方法,直观评价药材质量.
본문상시장중홍외광보(MIR)응용우육종용약재적질량평개화분석중.수선비교육종용약재분말양품급기3충주요활성성분제취물,포괄본을순총감(PeGs)、감로순(Man)화다당적홍외광보도차이,득출3충제취물적특정홍외흡수봉,학정약재양품각특정봉적강약여PeGs화Man함량고저적관계.연후용Spectrum v5.02연건계산득출불동파단내약재도보여량충제취물도보적홍외상사계수,통과SPSS11.0연건분석발현,약재PeGs적함량(y)화1800~1200cm 1범위내적홍외상사계수(x)정겁현저상관(R2=0.9927),회귀방정:y=19.846x+0.0623.동양,약재Man적함량(y)여1100～600cm-1파수범위적홍외상사계수(x)정겁현저상관(R2=0.9705),회귀방정:y=13.855x- 0.1714.인차,MIR가작위육종용약재질량정성화반정량분석적쾌속유효방법,직관평개약재질량.
In the present study, mid infrared spectroscopy(MIR) was attempted to apply for quality evaluation of C. deserticola. First of all, IR spectra of the herbal sample and its 3 main extracts including PeGs, Man and Polysaccharides were compared. Characteristic absorption peaks within different wave number range were found in different extracts. Then the authors managed to find the relationships between the intensity of the characteristic peaks and the relative content of the PeGs and Man in herbal sample. The IR spectra of the herbal samples were compared with PeGs and Man using Spectrum v5.02 software (Perkin Elmer) for IR Corrdations. It was found that the content of PeGs (y) and its IR Correlation (x) within herbal sample in the range of 1800～1200cm-1 were correlative (R2= 0.9927) by the SPSS 11.0 analysis, and the regression equation is y=19.846x + 0.0623. In the same way, the content of Man (y)is correlative with the IR Correlation (x) in the range of 1100～600cm-1 (R2 =0.9705), and the regression equation is y=13.855x-0.1714. Therefore, MIR spectroscopy is a fast and effective method in qualitative and semiquantitative analysis of quality evaluation of C. deserticola.