昆明医学院学报
昆明醫學院學報
곤명의학원학보
ACADEMIC JOURNAL OF KUNMING MEDICAL COLLEGE
2012年
5期
4-10
,共7页
陈鹏%李璠%何波%武鸿翔%杨建宇%张小超%沈志强
陳鵬%李璠%何波%武鴻翔%楊建宇%張小超%瀋誌彊
진붕%리번%하파%무홍상%양건우%장소초%침지강
老鹳草素%血小板聚集%细胞内钙%血小板-中性粒细胞相互作用
老鸛草素%血小闆聚集%細胞內鈣%血小闆-中性粒細胞相互作用
로관초소%혈소판취집%세포내개%혈소판-중성립세포상호작용
Geraniin%Platelet aggregation%Calcium%Platelet-neutrophil interactions
目的 研究老鹳草素抗血小板聚集作用及其对血小板与中性粒细胞之间相互作用的影响.方法 采用Born方法观测老鹳草素在体内和体外对家兔血小板聚集功能的影响,运用玫瑰花结实验测定血小板与中性粒细胞的黏附反应,以Fura-2-AM为荧光指示剂,检测老鹳草索对激活的血小板内钙水平的影响.结果 老鹳草素在体外呈浓度依赖性明显抑制花生四烯酸(AA)、腺苷二磷酸(ADP)及血小板活化因子(PAF)诱导的血小板聚集功能.其半数抑制浓度(IC50)分别为2.4、0.4和1.1μmol/L;5 mg/kg的老鹳草素灌胃亦明显抑制AA、ADP和PAF诱导的血小板聚集;老鹳草素显著降低洗涤血小板内钙离子浓度、减少细胞外钙内流及内钙释放,其IC50分别为71.9、84.9、62.9 μmol/L.老鹳草素明显阻抑凝血酶激活的血小板与中性粒细胞之间的黏附作用,并抑制肉豆蔻佛波醇(fMLP)激活的中性粒细胞上清液诱导的血小板聚集功能,其IC50分别为3.2和10.2 μmol/L.结论 老鹳草素明显抑制血小板聚集功能,降低血小板内钙水平,同时阻抑血小板与中性粒细胞之间的相互作用.
目的 研究老鸛草素抗血小闆聚集作用及其對血小闆與中性粒細胞之間相互作用的影響.方法 採用Born方法觀測老鸛草素在體內和體外對傢兔血小闆聚集功能的影響,運用玫瑰花結實驗測定血小闆與中性粒細胞的黏附反應,以Fura-2-AM為熒光指示劑,檢測老鸛草索對激活的血小闆內鈣水平的影響.結果 老鸛草素在體外呈濃度依賴性明顯抑製花生四烯痠(AA)、腺苷二燐痠(ADP)及血小闆活化因子(PAF)誘導的血小闆聚集功能.其半數抑製濃度(IC50)分彆為2.4、0.4和1.1μmol/L;5 mg/kg的老鸛草素灌胃亦明顯抑製AA、ADP和PAF誘導的血小闆聚集;老鸛草素顯著降低洗滌血小闆內鈣離子濃度、減少細胞外鈣內流及內鈣釋放,其IC50分彆為71.9、84.9、62.9 μmol/L.老鸛草素明顯阻抑凝血酶激活的血小闆與中性粒細胞之間的黏附作用,併抑製肉豆蔻彿波醇(fMLP)激活的中性粒細胞上清液誘導的血小闆聚集功能,其IC50分彆為3.2和10.2 μmol/L.結論 老鸛草素明顯抑製血小闆聚集功能,降低血小闆內鈣水平,同時阻抑血小闆與中性粒細胞之間的相互作用.
목적 연구로관초소항혈소판취집작용급기대혈소판여중성립세포지간상호작용적영향.방법 채용Born방법관측로관초소재체내화체외대가토혈소판취집공능적영향,운용매괴화결실험측정혈소판여중성립세포적점부반응,이Fura-2-AM위형광지시제,검측로관초색대격활적혈소판내개수평적영향.결과 로관초소재체외정농도의뢰성명현억제화생사희산(AA)、선감이린산(ADP)급혈소판활화인자(PAF)유도적혈소판취집공능.기반수억제농도(IC50)분별위2.4、0.4화1.1μmol/L;5 mg/kg적로관초소관위역명현억제AA、ADP화PAF유도적혈소판취집;로관초소현저강저세조혈소판내개리자농도、감소세포외개내류급내개석방,기IC50분별위71.9、84.9、62.9 μmol/L.로관초소명현조억응혈매격활적혈소판여중성립세포지간적점부작용,병억제육두구불파순(fMLP)격활적중성립세포상청액유도적혈소판취집공능,기IC50분별위3.2화10.2 μmol/L.결론 로관초소명현억제혈소판취집공능,강저혈소판내개수평,동시조억혈소판여중성립세포지간적상호작용.
Objective To investigate the effects of geraniin on platelet aggregation and platelet-neutrophil interactions.Methods Platelet aggregation,in vitro and ex vivo,was determined by use of Born's method,and the binding of thrombin-stimulated platelets to neutrophils was observed based on the rosette assay.Intracellular calcium concentration of platelets was measured by using Fura-2-AM.Results Geraniin in vitro significantly inhibited arachidonic acid (AA)-,adenosine diphosphate (ADP)-,or platelet activating factor (PAF)-induced platelet aggregation,in a concentration-dependent manner.The medium inhibitory concentrations (IC50) were 2.4,0.4 and 1.1 μmol/L,respectively.Intragastric geraniin at 5 mg/kg markedly suppressed platelet aggregation induced by AA,ADP,or PAF.Geraniin decreased the total rise of [Ca2+]i,Ca2+ release,and Ca2+ influx,in a concentration-dependant manner.The IC50 values were 71.9,84.9,and 62.9 μmol/L,respectively.Geraniin decreased the binding of thrombin-stimulated platelets to neutrophils,and significantly inhibited washed platelet aggregation stimulated by fMLP-activated neutrophils.The IC50 values were 3.2 and 10.2 μmol/L,respectively.Conclusion It is suggested that geraniin inhibited platelet aggregation in vitro and ex vivo,decreased the calcium mobilization of platelets,and suppressed the interactions between platelets and neutrophils.
