中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2008年
24期
17-19
,共3页
潘其重%陆日坚%梁增文%覃江
潘其重%陸日堅%樑增文%覃江
반기중%륙일견%량증문%담강
癌,肝细胞%核心结合因子α3亚基
癌,肝細胞%覈心結閤因子α3亞基
암,간세포%핵심결합인자α3아기
Carcinoma,hepatocellular%Core binding factor alpha 3 subunit
目的 探讨RUNX3基因及其蛋白在肝细胞癌(HCC)中的表达规律及其临床意义.方法 采用免疫组织化学SP法、反转录-聚合酶链反应(RT-PCR)等方法检测23例HCC患者肝癌组织和相应癌旁肝组织中RUNX3基因及其蛋白的表达情况.结果 肝癌组织中RUNX3基因及其蛋白的表达明显降低,阳性率分别为26.1%(6/23)和17.4%(4/23),明显低于癌旁肝组织的表达阳性率的91.3%(21/23)和87.0%(20/23),差异均有统计学意义(P均＜0.01).结论 RUNX3基因及其蛋白在肝癌组织中表达明显下调,提示RUNX3功能失活可能与HCC的发生和发展密切相关.
目的 探討RUNX3基因及其蛋白在肝細胞癌(HCC)中的錶達規律及其臨床意義.方法 採用免疫組織化學SP法、反轉錄-聚閤酶鏈反應(RT-PCR)等方法檢測23例HCC患者肝癌組織和相應癌徬肝組織中RUNX3基因及其蛋白的錶達情況.結果 肝癌組織中RUNX3基因及其蛋白的錶達明顯降低,暘性率分彆為26.1%(6/23)和17.4%(4/23),明顯低于癌徬肝組織的錶達暘性率的91.3%(21/23)和87.0%(20/23),差異均有統計學意義(P均＜0.01).結論 RUNX3基因及其蛋白在肝癌組織中錶達明顯下調,提示RUNX3功能失活可能與HCC的髮生和髮展密切相關.
목적 탐토RUNX3기인급기단백재간세포암(HCC)중적표체규률급기림상의의.방법 채용면역조직화학SP법、반전록-취합매련반응(RT-PCR)등방법검측23례HCC환자간암조직화상응암방간조직중RUNX3기인급기단백적표체정황.결과 간암조직중RUNX3기인급기단백적표체명현강저,양성솔분별위26.1%(6/23)화17.4%(4/23),명현저우암방간조직적표체양성솔적91.3%(21/23)화87.0%(20/23),차이균유통계학의의(P균＜0.01).결론 RUNX3기인급기단백재간암조직중표체명현하조,제시RUNX3공능실활가능여HCC적발생화발전밀절상관.
Objective To evaluatethe expression and significance of RUNX3 gene and protein in hepatocellular carcinoma.Method Reverse transcription-polymerase chain resction (RT-PCR) and immunoitistochemistry SP method were used to detect the expressions of RUNX3 gene and protein in 23 cases of hepatocellular carcinoma and their adjacent non-cancerous tissue.Results The expression of RUNX3 gene and protein were significantly reduced in hepatocellular carcinoma tissue (26.1% and 17.4% respectively),which were prominently lower than those in adjacent non-cancerous tissue:91.3% (21/23) and 87.0% (20/23),P＜0.01.Conclusion The lower-expression of RUNX3 gene and protein may play an important role in hepatocellular carcinoma.
