中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2011年
1期
33-37
,共5页
谢沛霖%薛晓东%张冀北%常飞
謝沛霖%薛曉東%張冀北%常飛
사패림%설효동%장기북%상비
神经生长因子/投药和剂量%胰岛素/投药和剂量%糖尿病并发症%伤口愈合/药物作用%烧伤/并发症%缺氧诱导因子1/代谢/药物作用%血管内皮生长因子类/代谢/药物作用
神經生長因子/投藥和劑量%胰島素/投藥和劑量%糖尿病併髮癥%傷口愈閤/藥物作用%燒傷/併髮癥%缺氧誘導因子1/代謝/藥物作用%血管內皮生長因子類/代謝/藥物作用
신경생장인자/투약화제량%이도소/투약화제량%당뇨병병발증%상구유합/약물작용%소상/병발증%결양유도인자1/대사/약물작용%혈관내피생장인자류/대사/약물작용
Nerve growth factor/AD%Insulin/AD%Diabetes complications/DT%Wound healing/DE%Burns/CO%Hypoxia-inducible factor 1/ME/DE%Vascular endothelial growth factors/ME/DE
目的 探讨局部应用神经生长因子(NGF)和胰岛素对1型糖尿病大鼠烫伤创面中缺氧诱导因子-1α(HIF-1α)和血管内皮细胞生长因子(VEGF)表达的影响及创面愈合机制.方法 雄性wistar大鼠腹腔注射STZ建立糖尿病模型60只,1月后在大鼠背部造成深Ⅱ度烫伤创面,将大鼠随机分为无干预组(B)、胰岛素治疗组(C)、NGF治疗组(D)、NGF联合胰岛素治疗组(E),并设立正常烫伤对照组(A),每组15只.观察伤后3、7、11、15、21 d各组创面愈合情况,检测创面组织HIF-1α和VEGF的表达.结果 E组创面愈合率第7天为[(25.33±2.32)%,P＜0.05],与其他各组相比较显著增加;C、D组创面愈合率较B组显著增加分别为[(16.68±1.95)%,(18.29±1.70)%,P＜0.05];D组自第15天起较A组显著增加[(54.84±3.60)%,P＜0.05];自第7天起E组HIF-1α和VEGF的表达显著低于其他各组(P＜0.05).结论 局部应用NGF联合胰岛素可加速创面愈合,其机制可能通过促进创面微血管再生,纠正局部缺氧.
目的 探討跼部應用神經生長因子(NGF)和胰島素對1型糖尿病大鼠燙傷創麵中缺氧誘導因子-1α(HIF-1α)和血管內皮細胞生長因子(VEGF)錶達的影響及創麵愈閤機製.方法 雄性wistar大鼠腹腔註射STZ建立糖尿病模型60隻,1月後在大鼠揹部造成深Ⅱ度燙傷創麵,將大鼠隨機分為無榦預組(B)、胰島素治療組(C)、NGF治療組(D)、NGF聯閤胰島素治療組(E),併設立正常燙傷對照組(A),每組15隻.觀察傷後3、7、11、15、21 d各組創麵愈閤情況,檢測創麵組織HIF-1α和VEGF的錶達.結果 E組創麵愈閤率第7天為[(25.33±2.32)%,P＜0.05],與其他各組相比較顯著增加;C、D組創麵愈閤率較B組顯著增加分彆為[(16.68±1.95)%,(18.29±1.70)%,P＜0.05];D組自第15天起較A組顯著增加[(54.84±3.60)%,P＜0.05];自第7天起E組HIF-1α和VEGF的錶達顯著低于其他各組(P＜0.05).結論 跼部應用NGF聯閤胰島素可加速創麵愈閤,其機製可能通過促進創麵微血管再生,糾正跼部缺氧.
목적 탐토국부응용신경생장인자(NGF)화이도소대1형당뇨병대서탕상창면중결양유도인자-1α(HIF-1α)화혈관내피세포생장인자(VEGF)표체적영향급창면유합궤제.방법 웅성wistar대서복강주사STZ건립당뇨병모형60지,1월후재대서배부조성심Ⅱ도탕상창면,장대서수궤분위무간예조(B)、이도소치료조(C)、NGF치료조(D)、NGF연합이도소치료조(E),병설립정상탕상대조조(A),매조15지.관찰상후3、7、11、15、21 d각조창면유합정황,검측창면조직HIF-1α화VEGF적표체.결과 E조창면유합솔제7천위[(25.33±2.32)%,P＜0.05],여기타각조상비교현저증가;C、D조창면유합솔교B조현저증가분별위[(16.68±1.95)%,(18.29±1.70)%,P＜0.05];D조자제15천기교A조현저증가[(54.84±3.60)%,P＜0.05];자제7천기E조HIF-1α화VEGF적표체현저저우기타각조(P＜0.05).결론 국부응용NGF연합이도소가가속창면유합,기궤제가능통과촉진창면미혈관재생,규정국부결양.
Objective To investigate the effects of local application of NGF combined with Insulin on wound healing with Ⅱ degree deep scald and the expression of HIF-1α and VEGF in diabetic rats. Methods 60 male Wistar rats were used in the study by intraperitoneal injection of streptozotocin (STZ). Deep partial thickness scalding was engendered on the back of the rats after one month. Then these rats were random divided into group B (scalding of the rats with diabetes, n = 15), C (insulin control, n = 15), D ( NGF control, n = 15) and E ( NGF and insulin supplementation, n = 15). And group A ( nomal control,n = 15) was created by normal rats of partial thickness scalding. The wound area, wound healing rate, and grey of HIF-1α and VEGF immunohistochemistry staining were determined on the 3rd, 7th, 15th, and 21st day post scalding days (PSDs). Results Compared to those in groups A, B, C and D, the wound healing rate in group E increased significantly since the 7th PSD [(25.33 ± 2. 32 ) %, P ＜ 0.05]. The wound healing rate in group C and D increased significantly compared with group B [( 16. 68 ± 1.95 ) %, ( 18.29±1. 70)%, P ＜0.05]. Compared to group A, the group D increased significantly since the 15th PSD [(54. 84 ±3.60)%, P ＜0. 05]. The expression of HIF-1α and VEGF in group E began to increase slower than other groups after the 7th day ( P ＜0. 05 ). Conclusion Local application of NGF combined with Insulin could be beneficial to the regeneration of capillary vessel in the burn wounds of the rats with diabetes,as well as to accelerate wound healing by redress anoxia and decrease the expression of HIF-1α and VEGF.
