中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2010年
5期
349-351
,共3页
王念跃%张岱%葛彦文%赵伟
王唸躍%張岱%葛彥文%趙偉
왕념약%장대%갈언문%조위
肝炎,乙型%病毒包膜蛋白质类%DNA%生物学标记
肝炎,乙型%病毒包膜蛋白質類%DNA%生物學標記
간염,을형%병독포막단백질류%DNA%생물학표기
Hepatitis B%Viral envilope proteins%DNA%Biological markers
目的 通过检测患者血清乙肝病毒外膜大蛋白(Hepatitis B virus large protein,HBVLP)、HBV-DNA以及乙肝病毒标志物(HBV-M),探讨HBV-LP对于反映体内乙肝病毒复制的意义.方法 对1886份血清标本采用酶联免疫吸附试验检测HBV-LP;化学发光免疫法测定HBV-M含量,PCR实时荧光定量检测HBV-DNA.结果 乙肝患者大蛋白检测结果与HBV-DNA检测结果差异无统计学意义(x2=1.142,P＞0.05).HBV-DNA拷贝数的对数值与HBV-LP吸光度呈正相关(r=0.487,P＜0.01),在不同HBV-DNA拷贝数组别间,HBV-LP A值差异有统计学意义(F=7.772,P＜0.01).在不同HBV-M模式中,HBV-DNA与HBV-LP的检测阳性率差异均无统计学意义.36例健康对照者,其HBV-DNA及HBV-LP测定结果均为阴性.结论 血清中HBV-LP的吸光密度值与HBV-DNA的拷贝数的对数有较好的相关性,HBV-LP能够反应HBV的复制情况.
目的 通過檢測患者血清乙肝病毒外膜大蛋白(Hepatitis B virus large protein,HBVLP)、HBV-DNA以及乙肝病毒標誌物(HBV-M),探討HBV-LP對于反映體內乙肝病毒複製的意義.方法 對1886份血清標本採用酶聯免疫吸附試驗檢測HBV-LP;化學髮光免疫法測定HBV-M含量,PCR實時熒光定量檢測HBV-DNA.結果 乙肝患者大蛋白檢測結果與HBV-DNA檢測結果差異無統計學意義(x2=1.142,P＞0.05).HBV-DNA拷貝數的對數值與HBV-LP吸光度呈正相關(r=0.487,P＜0.01),在不同HBV-DNA拷貝數組彆間,HBV-LP A值差異有統計學意義(F=7.772,P＜0.01).在不同HBV-M模式中,HBV-DNA與HBV-LP的檢測暘性率差異均無統計學意義.36例健康對照者,其HBV-DNA及HBV-LP測定結果均為陰性.結論 血清中HBV-LP的吸光密度值與HBV-DNA的拷貝數的對數有較好的相關性,HBV-LP能夠反應HBV的複製情況.
목적 통과검측환자혈청을간병독외막대단백(Hepatitis B virus large protein,HBVLP)、HBV-DNA이급을간병독표지물(HBV-M),탐토HBV-LP대우반영체내을간병독복제적의의.방법 대1886빈혈청표본채용매련면역흡부시험검측HBV-LP;화학발광면역법측정HBV-M함량,PCR실시형광정량검측HBV-DNA.결과 을간환자대단백검측결과여HBV-DNA검측결과차이무통계학의의(x2=1.142,P＞0.05).HBV-DNA고패수적대수치여HBV-LP흡광도정정상관(r=0.487,P＜0.01),재불동HBV-DNA고패수조별간,HBV-LP A치차이유통계학의의(F=7.772,P＜0.01).재불동HBV-M모식중,HBV-DNA여HBV-LP적검측양성솔차이균무통계학의의.36례건강대조자,기HBV-DNA급HBV-LP측정결과균위음성.결론 혈청중HBV-LP적흡광밀도치여HBV-DNA적고패수적대수유교호적상관성,HBV-LP능구반응HBV적복제정황.
Objective To explore the significance of serum hepatitis B virus large protein( HBVLP) , HBV-DNA and markers of hepatitis B virus (HBV-M)in the diagnosis of viral replication. Methods Serum HBV-DNA level was quantitantively detected using PCR Real-time polymerase chain reaction, HBVLP was detected by enzyme-linked immunosorbent assay (ELISA) and HBV markers expression were measured by chemiluminescence immunoassay method in 1886 cases of seurm. Results The results of hepatitis virus large protein (HBV-LP) detection and the detection results of HBV-DNA was no significant difference (x2 = 1. 142,P ＞0. 05). HBV-DNA logarithm of copies and A vaule of HBV-LP was a positive correlation (r = 0. 487, P ＜ 0.01 ). HBV-DNA copies of different groups was significantly different from HBV-LPA values ( F = 7. 772, P ＜ 0. 01 ). The results of HBV-LP and HBV-DNA detected in different patterns of HBV-M were not significantly different. In 36 healthy people,the detecting results of HBV-DNA and HBV-LP are negetive. Conclusion There is a good correlation between the copies of HBV-DNA and the levels of HBV-LP. HBV-LP expression can reflect the replication of HBV.
