中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2010年
10期
1389-1391
,共3页
刘细国%袁先厚%江普查%徐成仕
劉細國%袁先厚%江普查%徐成仕
류세국%원선후%강보사%서성사
信号转导和转录激活因子3%细胞周期蛋白D1%胶质瘤
信號轉導和轉錄激活因子3%細胞週期蛋白D1%膠質瘤
신호전도화전록격활인자3%세포주기단백D1%효질류
STAT3%Cyclin D1%Glioma
目的 探讨信号转导及转录激活因子3(STAT3)和细胞周期蛋白D1(Cyclin D1)表达与脑胶质瘤生物学行为的关系及意义.方法 用逆转录-聚合酶链反应(RT-PCR)和Western blot方法检测并比较68例不同级别胶质瘤和10例正常脑组织中STAT3和Cyclin D1的表达,并对两者表达做相关分析.结果 RT-PCR检测胶质瘤组织STAT3、Cyclin D1的mRNA表达量(2.23±0.32、2.18±0.26)均明显高于正常脑组织(0.53±0.14、0.48±0.11),Western blot检测胶质瘤组织STAT3、Cyclin D1的蛋白表达量(42.3±2.6)%、(45.4±1.8)%均明显高于正常脑组织(9.8±1.1)%、(10.4±0.9)%,其差异均有统计学意义(P＜0.05),STAT3和Cyclin D1的表达与胶质瘤级别呈正相关.结论 STAT3可能通过激活其下游靶基因Cyclin D1,引起胶质瘤细胞异常增殖和分化失控.
目的 探討信號轉導及轉錄激活因子3(STAT3)和細胞週期蛋白D1(Cyclin D1)錶達與腦膠質瘤生物學行為的關繫及意義.方法 用逆轉錄-聚閤酶鏈反應(RT-PCR)和Western blot方法檢測併比較68例不同級彆膠質瘤和10例正常腦組織中STAT3和Cyclin D1的錶達,併對兩者錶達做相關分析.結果 RT-PCR檢測膠質瘤組織STAT3、Cyclin D1的mRNA錶達量(2.23±0.32、2.18±0.26)均明顯高于正常腦組織(0.53±0.14、0.48±0.11),Western blot檢測膠質瘤組織STAT3、Cyclin D1的蛋白錶達量(42.3±2.6)%、(45.4±1.8)%均明顯高于正常腦組織(9.8±1.1)%、(10.4±0.9)%,其差異均有統計學意義(P＜0.05),STAT3和Cyclin D1的錶達與膠質瘤級彆呈正相關.結論 STAT3可能通過激活其下遊靶基因Cyclin D1,引起膠質瘤細胞異常增殖和分化失控.
목적 탐토신호전도급전록격활인자3(STAT3)화세포주기단백D1(Cyclin D1)표체여뇌효질류생물학행위적관계급의의.방법 용역전록-취합매련반응(RT-PCR)화Western blot방법검측병비교68례불동급별효질류화10례정상뇌조직중STAT3화Cyclin D1적표체,병대량자표체주상관분석.결과 RT-PCR검측효질류조직STAT3、Cyclin D1적mRNA표체량(2.23±0.32、2.18±0.26)균명현고우정상뇌조직(0.53±0.14、0.48±0.11),Western blot검측효질류조직STAT3、Cyclin D1적단백표체량(42.3±2.6)%、(45.4±1.8)%균명현고우정상뇌조직(9.8±1.1)%、(10.4±0.9)%,기차이균유통계학의의(P＜0.05),STAT3화Cyclin D1적표체여효질류급별정정상관.결론 STAT3가능통과격활기하유파기인Cyclin D1,인기효질류세포이상증식화분화실공.
Objective To investigate the relationship between the signal transducer, activator of transcription 3 (STAT3), Cyclin D1 (Cyclin D1 ) expression and the biological behavior of gliomas and the significance. Methods By using reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting, the expression of STAT3 and Cyclin D1 was detected in 68 patients with different grades of gliomas and 10 normal brain tissues, and the relationship of STAT3 and survivin expression was analyzed. Results RT-PCR revealed that the expression of STAT3, Cyclin D1 mRNA in gliomas was ( 2.23 ± 0. 32 ) and (2. 18 ±0.26) respectively, which was significantly higher than in normal brain tissue (0.53 ±0. 14, 0.48 ±0. 11 respectively). Western blotting demonstrated that the expression of STAT3 and Cyclin D1 protein in gliomas was (42.3 ±2.6)% and (45.4 ± 1.8)% respectively, which was significantly higher than in normal brain tissue[(9.8 ± 1.1 ) % and ( 10.4 ± 0.9) % ,P ＜ 0.05]. STAT3 and cyclin D1 expression was correlated with glioma grade. Conclusion STAT3 may activate its downstream target genes Cyclin D1, causing abnormal proliferation and differentiation out of control in glioma cells.
