CAJ | 학술논문

最近,我们研究发现大鼠胸腺和淋巴结淋巴细胞中存在降钙素基因相关肽(CGRP)样免疫反应活性.应用人工合成的可特异扩增降钙素/降钙素基因相关肽基因部分片断的寡核苷酸引物,通过逆转录-多聚酶链反应(RT-PCR),检测在大鼠脊髓背根神经节、胸腺细胞及肠系膜淋巴结淋巴细胞中是否存在CGRP mRNA,进一步研究大鼠淋巴细胞能否合成CGRP.结果显示,通过RT-PCR从脊髓背根神经节(阳性对照)、胸腺和淋巴结淋巴细胞样品中,都检测到预期的90个碱基对的CGRP cDNA片断.这提示,CGRP除了是一种重要的神经递质外,也可在胸腺细胞和淋巴结淋巴细胞合成.此种CGRP可能与感觉神经源的一样,是免疫和神经系统的共用配基之一.淋巴细胞源的CGRP可能起着免疫细胞调节因子的作用.
최근,아문연구발현대서흉선화림파결림파세포중존재강개소기인상관태(CGRP)양면역반응활성.응용인공합성적가특이확증강개소/강개소기인상관태기인부분편단적과핵감산인물,통과역전록-다취매련반응(RT-PCR),검측재대서척수배근신경절、흉선세포급장계막림파결림파세포중시부존재CGRP mRNA,진일보연구대서림파세포능부합성CGRP.결과현시,통과RT-PCR종척수배근신경절(양성대조)、흉선화림파결림파세포양품중,도검측도예기적90개감기대적CGRP cDNA편단.저제시,CGRP제료시일충중요적신경체질외,야가재흉선세포화림파결림파세포합성.차충CGRP가능여감각신경원적일양,시면역화신경계통적공용배기지일.림파세포원적CGRP가능기착면역세포조절인자적작용.
We recently found that calcitonin gene-related peptide (CGRP)-like immunoreactivity was present in lymphocytes of rat thymus and lymph node. In order to investigate whether these cells were capable of synthesizing CGRP, CGRP mRNA of rat dorsal root ganglia, thymocytes and mesenteric lymph node lymphocytes were determined by reverse transcription and polymerase chain reaction (RT-PCR) utilizing synthetic oligonucleotides bracketing a portion of the calcitonin/CGRP gene. A discrete band of the expected size of 90 base pairs was found in the dorsal root ganglia (positive control), and in both thymocytes and mesenteric lymph node lymphocytes. These data strongly suggest that CGRP is not only an important neuropeptide, but it is also synthesized in lymphocytes of both thymus and lymph nodes, which is identical to that in sensory neurons. CGRP from lymphocytes may act as an immunomodulator and serve as a common ligand in immune and nervous systems.