CAJ | 학술논문

报导了一个分子标记连锁图的快速构建方法.通过对水稻(Oryza sativa L.)"安农S-1"和"南京11"的F2分离群体的AFLP分析找到了142个AFLP标记,用这142个AFLP标记以及已定位的25个SSR标记和5个RFLP标记构建了水稻12个染色体的分子标记连锁图,该图覆盖水稻基因组的1 537.4 cM,相邻标记间的平均间距为9.0 cM,这是在国内建立的第一张AFLP标记连锁图.在建立连锁图谱的同时把一个新基因tms5 (水稻温敏核不育基因)定位在第2染色体上.
보도료일개분자표기련쇄도적쾌속구건방법.통과대수도(Oryza sativa L.)"안농S-1"화"남경11"적F2분리군체적AFLP분석조도료142개AFLP표기,용저142개AFLP표기이급이정위적25개SSR표기화5개RFLP표기구건료수도12개염색체적분자표기련쇄도,해도복개수도기인조적1 537.4 cM,상린표기간적평균간거위9.0 cM,저시재국내건립적제일장AFLP표기련쇄도.재건립련쇄도보적동시파일개신기인tms5 (수도온민핵불육기인)정위재제2염색체상.
In this study, we reported the repaid construction of a molecular marker linkage map of rice (Oryza sativa L.). An F2 population from the cross between Annong S-1 and Nanjing 11 was used to construct a genetic linkage map of rice. Total of 142 newly screened AFLP markers and 30 anchor markers (25 SSR markers and 5 RFLP markers) were mapped on the 12 chromosomes covering 1 537.4 cM of rice genome. The average interval between these markers was 9.0 cM. The total work which usually was finished in more than one year was finished within only 3 months by one person. This is the first plant AFLP map developed in China. A new thermosensitive genic male sterile gene in rice, tms5, was tigged and mapped onto chromosome 2 during the development of the linkage map.