CAJ | 학술논문

目的:通过研究异常瘢痕患者外周血单个核细胞HLA-DR,-DQ,-DP及CD1a分子的表达及分布,探讨免疫遗传因素在异常瘢痕形成中的作用.方法:采用免疫细胞化学方法检测10例瘢痕疙瘩患者、10例增生性瘢痕患者、10例扁平瘢痕患者和10例正常人外周血单个核细胞HLA-Ⅱ类分子(HLA-DR,-DQ,-DP)及CD1a分子的表达及分布.结果:①HLA-DR+,-DQ+,-DP+及CD1a+细胞数量在瘢痕疙瘩患者、增生性瘢痕患者、扁平瘢痕患者和正常人各组间差异无显著性意义(P＞0.05).②瘢痕疙瘩和增生性瘢痕组外周血HLA-DR+细胞(813.16±62.77,883.11±146.71;F=10.613,P＜0.01,-DQ+细胞(420.12±94.25,439 24±146.65;F=5.143,P＜0.01),-DP+细胞(259.40±57.43,271.93±70.38;F=9.177,P＜0.01)及CD1a+细胞(580.15±108.48,648.94±185.46;F=8.581,P＜0.01)的积分光密度高于扁平瘢痕和正常人组外周血HLA-DR+(636.22±133.95,597.88±166.36),-DQ+(302.77±89.77,308.57±44.05),-DP+(192.96±51 32,159.01 ±31.99)及CD1 a+(423.53±142.36,368.67±117.18)细胞的积分光密度,表明瘢痕疙瘩患者和增生性瘢痕患者组外周血单个核细胞HLA-DR,-DQ,-DP及CD1a蛋白含量高于扁平瘢痕患者和正常人组.结论:瘢痕疙瘩患者和增生性瘢痕患者外周血HLA-DR,-DQ,-DP及CD1a蛋白含量的增高提示瘢痕的超常增生可能与机体免疫遗传因素有关.
목적:통과연구이상반흔환자외주혈단개핵세포HLA-DR,-DQ,-DP급CD1a분자적표체급분포,탐토면역유전인소재이상반흔형성중적작용.방법:채용면역세포화학방법검측10례반흔흘탑환자、10례증생성반흔환자、10례편평반흔환자화10례정상인외주혈단개핵세포HLA-Ⅱ류분자(HLA-DR,-DQ,-DP)급CD1a분자적표체급분포.결과:①HLA-DR+,-DQ+,-DP+급CD1a+세포수량재반흔흘탑환자、증생성반흔환자、편평반흔환자화정상인각조간차이무현저성의의(P＞0.05).②반흔흘탑화증생성반흔조외주혈HLA-DR+세포(813.16±62.77,883.11±146.71;F=10.613,P＜0.01,-DQ+세포(420.12±94.25,439 24±146.65;F=5.143,P＜0.01),-DP+세포(259.40±57.43,271.93±70.38;F=9.177,P＜0.01)급CD1a+세포(580.15±108.48,648.94±185.46;F=8.581,P＜0.01)적적분광밀도고우편평반흔화정상인조외주혈HLA-DR+(636.22±133.95,597.88±166.36),-DQ+(302.77±89.77,308.57±44.05),-DP+(192.96±51 32,159.01 ±31.99)급CD1 a+(423.53±142.36,368.67±117.18)세포적적분광밀도,표명반흔흘탑환자화증생성반흔환자조외주혈단개핵세포HLA-DR,-DQ,-DP급CD1a단백함량고우편평반흔환자화정상인조.결론:반흔흘탑환자화증생성반흔환자외주혈HLA-DR,-DQ,-DP급CD1a단백함량적증고제시반흔적초상증생가능여궤체면역유전인소유관.