CAJ | 학술논문

以1-(4-异硫氰苄基)乙烯基二胺-N,N,N′,N′-四乙酸为双功能螯合剂,偶联Cd2+与血清蛋白质分子, 合成了Cd2+人工抗原.利用荧光光谱分析了Cd2+人工合成抗原的荧光特性并得到合成反应的偶联比为11∶ 1～16∶ 1;通过荧光相图分析表明, Cd2+人工抗原合成过程中血清蛋白质的折叠状态符合"二态模型";偏振荧光光谱检测结果显示, Cd2+人工抗原合成中血清蛋白质色氨酸残基的微区构象发生一定变化,导致载体蛋白质构象改变.
이1-(4-이류청변기)을희기이알-N,N,N′,N′-사을산위쌍공능오합제,우련Cd2+여혈청단백질분자, 합성료Cd2+인공항원.이용형광광보분석료Cd2+인공합성항원적형광특성병득도합성반응적우련비위11∶ 1～16∶ 1;통과형광상도분석표명, Cd2+인공항원합성과정중혈청단백질적절첩상태부합"이태모형";편진형광광보검측결과현시, Cd2+인공항원합성중혈청단백질색안산잔기적미구구상발생일정변화,도치재체단백질구상개변.
Artificial antigen Cd-IEDTA-BSA (HSA) was successfully synthesized by the bifunctional chelating agent IEDTA (isothiocyanobenzylethyl enediamine tetraacetic acid) coupling with cadmium and protein carrier. The fluorescence characteristics of antigen were determined by fluorescence spectrophotometry and the coupling ratio 11∶ 1-16∶ 1 was obtained for the coupling reaction. The folding status of serum albumin during the synthesis process of artificial antigen Cd-IEDTA-BSA (HSA) was analyzed using "fluorescence phase diagram", and the result shows that it was conformed to the "all-or-none" pattern. The polarization fluorescence spectra show that the microenvironments of tryptophan residues in carrier protein have some changes to cause changes in protein conformation.