CAJ | 학술논문

为了有效利用鱼类废弃物,从草鱼鳔中提取酸溶性胶原(ASC)和酶溶性胶原(PSC)并对其部分性质进行研究.ASC和PSC的提取得率以干基计分别为4.7%和11.1%;ASC和PSC的紫外光谱表明,最大吸收峰都接近于230nm:氨基酸组成分析表明,ASC和PSC中羟脯氨酸和羟赖氨酸的总含量分别为16.87%和17.55%;十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)图谱显示草鱼鳔胶原至少由两条小同的α链(α_1和α_2)组成,与Ⅰ型胶原相似;ASC和PSC热变性温度(T_d)分别为33.0、34.8℃,比猪皮胶原的热变性温度(37℃)低3～4℃.结果表明,草鱼鳔胶原有可能替代或作为补充陆生动物胶原广泛应用于工业领域.
위료유효이용어류폐기물,종초어표중제취산용성효원(ASC)화매용성효원(PSC)병대기부분성질진행연구.ASC화PSC적제취득솔이간기계분별위4.7%화11.1%;ASC화PSC적자외광보표명,최대흡수봉도접근우230nm:안기산조성분석표명,ASC화PSC중간포안산화간뢰안산적총함량분별위16.87%화17.55%;십이완기류산납-취병희선알응효전영(SDS-PAGE)도보현시초어표효원지소유량조소동적α련(α_1화α_2)조성,여Ⅰ형효원상사;ASC화PSC열변성온도(T_d)분별위33.0、34.8℃,비저피효원적열변성온도(37℃)저3～4℃.결과표명,초어표효원유가능체대혹작위보충륙생동물효원엄범응용우공업영역.
In order to make more effective use offish waste materials, acid-soluble and pepsin-soluble collagens (ASC and PSC) were extracted from swim bladders of grass carp and properties of ASC and PSC were investigated. The yields of ASC and PSC were 4.7% and 11.1%, respectively, on the basis of dry weight. UV spectra of ASC and PSC from grass carp exhibited the maximum absorption peak at 230 nm. Amino acid profiles of ASC and PSC revealed that contents of hydroxyproline and proline were 16.87% and 17.55%, respectively. SDS-PAGE patterns indicated that both ASC and PSC were composed of at least two different α-chains (α_1 and α_2), which was similar to porcine type Ⅰ collagen. The denaturation temperatures (T_d) of ASC and PSC were 33.0 ℃ and 34.8 ℃, respectively, about 3-4 ℃ lower than that of porcine skin collagen (37 ℃). These results suggested that collagens from swim bladders of grass carp had potential as an alternative or a supplementary source of collagen from land vertebrates being used in different fields for industrial purposes.