CAJ | 학술논문

背景:雷帕霉素全身用药可明确抑制角膜移植后植片新生血管的生长,但雷帕霉素滴眼剂能否起到相同或者类似的作用尚有待验证.目的:观察雷帕霉索滴眼剂抑制大鼠角膜新生血管生长过程中血管内皮生长因子及炎症因子的作用.方法:角膜缝线法建立SD大鼠左眼角膜新生血管模型,以抽签法随机分为生理盐水对照组与雷帕霉素滴眼剂组.各组均术后第1天开始用药,4次/d,1滴,次.每日行裂隙灯显微镜检查,术后第3,7,14天测量角膜新生血管的长度,计算新生血管面积.14 d后切取角膜行组织学检查,并采用免疫组织化学染色法检测血管内皮生长因子以及白细胞介素2受体α的表达.结果与结论:生理盐水对照组不同时间点角膜新生血管面积均高于雷帕霉素滴眼剂组(P=0),说明雷帕霉索滴眼剂可抑制缝线后角膜新生血管的生长.与生理盐水对照组比较,雷帕霉素滴眼剂组角膜上皮层、浅基质层、新生血管内皮、角膜缝线处上皮以及浸润的炎性细胞内血管内皮生长因子及白细胞介素2受体α表达明显降低,病理切片上炎性细胞浸润减少.结果提示雷帕霉素滴眼剂抑制角膜新生血管生长,可能通过降低血管内皮生长因子的表达及抑制炎性细胞的分化浸润从而抑制新生血管的生长.
배경:뢰파매소전신용약가명학억제각막이식후식편신생혈관적생장,단뢰파매소적안제능부기도상동혹자유사적작용상유대험증.목적:관찰뢰파매색적안제억제대서각막신생혈관생장과정중혈관내피생장인자급염증인자적작용.방법:각막봉선법건립SD대서좌안각막신생혈관모형,이추첨법수궤분위생리염수대조조여뢰파매소적안제조.각조균술후제1천개시용약,4차/d,1적,차.매일행렬극등현미경검사,술후제3,7,14천측량각막신생혈관적장도,계산신생혈관면적.14 d후절취각막행조직학검사,병채용면역조직화학염색법검측혈관내피생장인자이급백세포개소2수체α적표체.결과여결론:생리염수대조조불동시간점각막신생혈관면적균고우뢰파매소적안제조(P=0),설명뢰파매색적안제가억제봉선후각막신생혈관적생장.여생리염수대조조비교,뢰파매소적안제조각막상피층、천기질층、신생혈관내피、각막봉선처상피이급침윤적염성세포내혈관내피생장인자급백세포개소2수체α표체명현강저,병리절편상염성세포침윤감소.결과제시뢰파매소적안제억제각막신생혈관생장,가능통과강저혈관내피생장인자적표체급억제염성세포적분화침윤종이억제신생혈관적생장.
BACKGROUND:Systemic administration of rapamycin has been demonstrated to inhibit graft neovascularization following corneal transplantation.However,the effect of rapamycin eye drops remains unknown.OBJECTIVE:To explore the role of vascular endothelial growth factor(VEGF)and inflammatory factor during the inhibition of rapamycin eye drops on corneal neovascularization.MEHODS:Comeal neovascularization in the left eye was induced by suture method,and the 12 SD rats were randomly assigned to normal saline and rapamycin groups.Drugs were delivered.1 drop each,4 times daily since 1 day after operation.Rat corneas were checked by slit lamp microscope daily.The length of corneal neovascularization was measured at days 3,7,and 14 to calculate the area of Corneal neovasculadzation.The expression of VEGF and intedeukin-2 receptor α(IL-2Rα)was detected by immunohistochemicel techniques at 14 days.RESULTS AND CONCLUSION:Comesl neovascularization area of normal saline group was significantly greater than rapamycin group at each time point(P=0),indicating that rapamydn eye drop inhibited comeal neovascularization following suture.Compared with normal saline group,the expression of VEGF and IL-2Rα was significantly decreased in corneal epithelia,stroma.comesl neovascular endothelium and epithelium at the suture site,as well as inflammatory cells of rapamycin group.Moreover.the inflemmatory cellinfiItrete was reduced in the pathological sections of rapamycin group.Results show that rapamvdn eye drops inhibit corneal neovascularization,possibly by reducing the expression of VEGF and IL-2Rα.