CAJ | 학술논문

为了解上海地区规模化猪场分离的大肠埃希菌产超广谱β-内酰胺酶（ESBLs）的分离率、耐药性和基因型，收集2010年9月-2011年3月从上海地区10个规模化养猪场分离的296株大肠埃希菌，采用微量肉汤稀释法进行药敏试验以及PCR技术分别检测TEM、CTX-M、OXA和SHV基因。在296株大肠埃希菌中，52株大肠埃希菌产ESBLs，产酶率为17．6％。在52株ESBLs菌株中，46株为TEM型，占88．5％；15株为CTX—M型，占28．8％；11株为同时携带TEM型和CTX—M型，占21．2％。大肠埃希菌产ESBLs株对10种常用抗菌药物的耐药性较为严重，而且存在多重耐药；上海地区猪源大肠埃希菌产ESBLs株流行的主要基因型为TEM型和CTX—M型。
위료해상해지구규모화저장분리적대장애희균산초엄보β-내선알매（ESBLs）적분리솔、내약성화기인형，수집2010년9월-2011년3월종상해지구10개규모화양저장분리적296주대장애희균，채용미량육탕희석법진행약민시험이급PCR기술분별검측TEM、CTX-M、OXA화SHV기인。재296주대장애희균중，52주대장애희균산ESBLs，산매솔위17．6％。재52주ESBLs균주중，46주위TEM형，점88．5％；15주위CTX—M형，점28．8％；11주위동시휴대TEM형화CTX—M형，점21．2％。대장애희균산ESBLs주대10충상용항균약물적내약성교위엄중，이차존재다중내약；상해지구저원대장애희균산ESBLs주류행적주요기인형위TEM형화CTX—M형。
To investigate the isolation rate, antibiotic susceptibility and ESBLs＇ genotypes of Escherichia coli strains isolated from ten large - scale pig farms in Shanghai, 296 strains of Escherichia coli were collected from September 2010 to March 2011. Susceptibility testing was performed by broth microdilution, and the genotypes of TEM, CTX -M, OXA and SHV genes were analyzed by PCR. There were 52 strains expressing ESBLs and the isolated rate was 17.6%. There were 46 strains expressing TEM gene （ 88.5% ） , 15 strains expressing CTX - M gene （28.8%） , 11 strains expressing both TEM gene and CTX - M gene （21.2%） among 52 strains. The susceptibility testing against 10 antimicrobial agents of ESBLs - producing Escherichia coli was serious and multidrug resistant. This study showed that TEM type and CTX - M type were predominant genotypes of ESBLs in Escherichia coli strains isolated from pigs.