国际生物医学工程杂志
國際生物醫學工程雜誌
국제생물의학공정잡지
INTERNATIONAL JOURNAL OF BIOMEDICAL ENGINEERING
2011年
3期
150-153
,共4页
糖尿病%大鼠%海马前穿通纤维-齿状回通路%突触可塑性降低%长时程增强
糖尿病%大鼠%海馬前穿通纖維-齒狀迴通路%突觸可塑性降低%長時程增彊
당뇨병%대서%해마전천통섬유-치상회통로%돌촉가소성강저%장시정증강
Diabetes%Rats%Perforant path-dentate gyrus pathway%Reduced synaptic plasticity%Long term-potentiation
目的 研究大鼠在患1型和2型糖尿病后,对在体海马前穿通纤维-齿状回通路(PP-DG)的突触可塑性造成的影响.方法 将70只SD大鼠(180±20)g随机分成3组:对照组、1型糖尿病模型组、2型糖尿病模型组.在水迷宫测试后,在每个模型组中选出空间记忆能力较差的15只大鼠,研究糖尿病引起的海马PP-DC通路双脉冲易化(PPF)、长时程增强(LTP)等突触可塑性的变化.结果 大鼠在患1型和2型糖尿病后,与对照组相比,海马LTP的形成均被显著抑制(P<0.05),并且1型糖尿病对LTP的抑制作用更大;糖尿病抑制了海马PPF的幅度(P<0.05),并减小了PPF的易化宽度.结论 1型和2型糖尿病对大鼠海马PP-DG通路的突触可塑性造成了破坏,这与之前水迷宫行为学测试的结果相对应.
目的 研究大鼠在患1型和2型糖尿病後,對在體海馬前穿通纖維-齒狀迴通路(PP-DG)的突觸可塑性造成的影響.方法 將70隻SD大鼠(180±20)g隨機分成3組:對照組、1型糖尿病模型組、2型糖尿病模型組.在水迷宮測試後,在每箇模型組中選齣空間記憶能力較差的15隻大鼠,研究糖尿病引起的海馬PP-DC通路雙脈遲易化(PPF)、長時程增彊(LTP)等突觸可塑性的變化.結果 大鼠在患1型和2型糖尿病後,與對照組相比,海馬LTP的形成均被顯著抑製(P<0.05),併且1型糖尿病對LTP的抑製作用更大;糖尿病抑製瞭海馬PPF的幅度(P<0.05),併減小瞭PPF的易化寬度.結論 1型和2型糖尿病對大鼠海馬PP-DG通路的突觸可塑性造成瞭破壞,這與之前水迷宮行為學測試的結果相對應.
목적 연구대서재환1형화2형당뇨병후,대재체해마전천통섬유-치상회통로(PP-DG)적돌촉가소성조성적영향.방법 장70지SD대서(180±20)g수궤분성3조:대조조、1형당뇨병모형조、2형당뇨병모형조.재수미궁측시후,재매개모형조중선출공간기억능력교차적15지대서,연구당뇨병인기적해마PP-DC통로쌍맥충역화(PPF)、장시정증강(LTP)등돌촉가소성적변화.결과 대서재환1형화2형당뇨병후,여대조조상비,해마LTP적형성균피현저억제(P<0.05),병차1형당뇨병대LTP적억제작용경대;당뇨병억제료해마PPF적폭도(P<0.05),병감소료PPF적역화관도.결론 1형화2형당뇨병대대서해마PP-DG통로적돌촉가소성조성료파배,저여지전수미궁행위학측시적결과상대응.
Objective To study the effects of diabetes on hippocampal synaptic plasticity in perforant path-dentate gyrus pathway (PP-DG) in rats. Methods 70 SD rats( 180±20) g were divided into 3 groups at random: control group, type1 diabetes group (DM1)and type2 diabetes group (DM2). After Morris water maze test, 15 rats that showed worse spatial memory ability were selected in each model group to investigate the variation of paired-pulse facilitation (PPF) and the range of synaptic plasticity. Field potentials were recorded in the dentate gyrus of the dorsal hippocampus by stimulating the perforant path. Results Contrast to the control group, diabetic rats' hippocampal LTP were depressed (P<0.05), and type1 diabetic rats' LTP reduced much more. Diabetic rats' PPF ratio was reduced contrast to the control group (P<0.05). Conclusion Type1 and type2 diabetes impaired synaptic plasticity of hippocampal PP-DG pathway in rats, which conformed the results of water maze test.
