CAJ | 학술논문

目的探讨3-(5'-羟甲基-2’-呋喃基)-1-苯甲基吲唑(YC-1)对乏氧入肺腺癌A549细胞的放射增敏作用.方法用噻唑蓝比色法检测YC-1对细胞增殖活性的影响,以及YC-1对细胞作用24h的20％药物抑制浓度(IC20).细胞克隆形成实验计算细胞存活分数,多靶单击模型拟合细胞存活曲线并计算放射增敏比(SER).流式细胞仪检测细胞凋亡率和细胞周期.结果 YC-1对A549细胞有增殖抑制作用,在一定剂量范围内呈时间一剂量依赖性.在常氧和乏氧培养24h下aIC20分别为16.7 μmol/L和39.2 μmol/L.乏氧加YC-1组的SERD0=1.11,SERDq=1.26.在乏氧培养下,YC-1联合单次2 Gy放射能明显促进A549细胞凋亡和G2 +M期阻滞[(30.17±1.21)％∶(15.44±0.96)％,P=0.000和(21.56±0.47)％∶(6.16±0.16)％,P=0.000].结论 YC-1对乏氧的A549细胞有放射增敏作用.
목적 탐토3-(5'-간갑기-2’-부남기)-1-분갑기신서(YC-1)대핍양입폐선암A549세포적방사증민작용.방법 용새서람비색법검측YC-1대세포증식활성적영향,이급YC-1대세포작용24h적20％약물억제농도(IC20).세포극륭형성실험계산세포존활분수,다파단격모형의합세포존활곡선병계산방사증민비(SER).류식세포의검측세포조망솔화세포주기.결과 YC-1대A549세포유증식억제작용,재일정제량범위내정시간일제량의뢰성.재상양화핍양배양24h하aIC20분별위16.7 μmol/L화39.2 μmol/L.핍양가YC-1조적SERD0=1.11,SERDq=1.26.재핍양배양하,YC-1연합단차2 Gy방사능명현촉진A549세포조망화G2 +M기조체[(30.17±1.21)％∶(15.44±0.96)％,P=0.000화(21.56±0.47)％∶(6.16±0.16)％,P=0.000].결론 YC-1대핍양적A549세포유방사증민작용.
Objective To investigate the radiosensitizing effect of 3-(5'-hydroxy-2'-furyl)-1-benzyl indazole ( YC-1 ) on hypoxic human adenocarcinoma cell line A549.Methods MTT assay was used to test the inhibitory effect of YC-1 on proliferation of A549 cells.Clonogenic assay was performed to determine the radiosensitizing effect of YC-1 on hopxic A549 cells.Single-hit multi-target model was used to plot survival curve and calculate sensitization enhancement ratio (SER).The cell cycle and apoptosis were measured by flow cytometry.Results The proliferation of A549 cells was inhibited by YC-1 in a time-dose-dependent manner.In normoxic and hypoxic cells,the IC20 was 16.7 μmol/L and 39.2 μmol/L at 24 h,respectively.In the group of hypoxia plus YC-1,SERD0 and SERDq were 1.11 and 1.26,respectively.In hypoxia,YC-1combined with 2 Gy irradiation could induce cell apoptosis and prolong G2 + M phase arrest ( ( 30.17 ±1.21 )％ ∶ ( 15.44 ±0.96) ％,P =0.000; (21.56 ±0.47 )％ ∶ (6.16 ±0.16)％,P =0.000).Concinsions YC-1 could enhance the radiosensitivity of hypoxic A549 cells.