中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2011年
4期
325-330
,共6页
刘文军%梁灼萍%陈祖尧%覃纲%黄英%黎万荣
劉文軍%樑灼萍%陳祖堯%覃綱%黃英%黎萬榮
류문군%량작평%진조요%담강%황영%려만영
鼻炎,变应性,常年性%哮喘%基质金属蛋白酶9%肿瘤坏死因子α%嗜酸细胞%中性白细胞
鼻炎,變應性,常年性%哮喘%基質金屬蛋白酶9%腫瘤壞死因子α%嗜痠細胞%中性白細胞
비염,변응성,상년성%효천%기질금속단백매9%종류배사인자α%기산세포%중성백세포
Rhinitis,allergic,perennial%Asthma%Matrix metalloproteinase 9%Tumor necrosis factor-alpha%Eosinophils%Neutrophils
目的 通过建立变应性鼻炎(allergic rhinitis,AR)和支气管哮喘(简称哮喘,asthma,AS)动物模型,探讨基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)和肿瘤坏死因子α(tumor necrosis factor,TNF-α)在上下呼吸道炎性反应一致性中的作用及机制.方法 以卵清蛋白辅以氢氧化铝致敏并激发制成AR和AS大鼠模型.HE染色和甲苯胺蓝染色分别检测AR和AS大鼠模型鼻黏膜及肺组织中嗜酸粒细胞、肥大细胞的表达,免疫组化SP法检测上述组织中MMP-9和TNF-α的表达,分析嗜酸粒细胞、肥大细胞、MMP-9和TNF-α表达与上下呼吸道炎性反应的关系.采用SPSS13.0软件对数据进行统计学分析.结果 MMP-9阳性细胞数在AR组鼻黏膜和肺组织中分别为 (154.8±12.0)、(124.0±8.2)个,在AR对照组分别为(43.2±7.6)、(34.5±5.0)个,差异有统计学意义(t值分别为24.260、29.525,P值均＜0.05);MMP-9阳性细胞数在AS组鼻黏膜和肺组织中分别为(149.9±11.7)、(120.1±7.3)个,在AS对照组分别为(48.6±7.6)、(39.1±5.2)个,差异有统计学意义(t值分别为22.929、28.530,P值均＜0.05).TNF-α阳性细胞数在AR组鼻黏膜和肺组织中分别为(188.8±17.0)、(134.8±7.9)个,在AR对照组分别为(57.6±23.3)、(40.3±8.2)个,差异有统计学意义(t值分别为13.836、26.220,P值均＜0.05);TNF-α阳性细胞数在AS组鼻黏膜和肺组织中分别为(179.2±15.4)、(153.5±10.1)个,在AS对照组分别为(70.5±33.1)、(33.8±14.0)个,差异有统计学意义(t值分别为9.412、21.858,P值均＜0.05).MMP-9与TNF-α在AR组鼻黏膜及肺组织中的表达分别呈正相关(r值分别为0.893和0.700,P值分别为0.001和0.024),二者在AS组鼻黏膜或肺组织中的表达分别呈正相关(r值分别为0.692和0.644,P值分别为0.027和0.044).结论 上下呼吸道炎性反应具有一致性,MMP-9和TNF-α可能在上下呼吸道炎性反应一致性中发挥重要作用.
目的 通過建立變應性鼻炎(allergic rhinitis,AR)和支氣管哮喘(簡稱哮喘,asthma,AS)動物模型,探討基質金屬蛋白酶9(matrix metalloproteinase-9,MMP-9)和腫瘤壞死因子α(tumor necrosis factor,TNF-α)在上下呼吸道炎性反應一緻性中的作用及機製.方法 以卵清蛋白輔以氫氧化鋁緻敏併激髮製成AR和AS大鼠模型.HE染色和甲苯胺藍染色分彆檢測AR和AS大鼠模型鼻黏膜及肺組織中嗜痠粒細胞、肥大細胞的錶達,免疫組化SP法檢測上述組織中MMP-9和TNF-α的錶達,分析嗜痠粒細胞、肥大細胞、MMP-9和TNF-α錶達與上下呼吸道炎性反應的關繫.採用SPSS13.0軟件對數據進行統計學分析.結果 MMP-9暘性細胞數在AR組鼻黏膜和肺組織中分彆為 (154.8±12.0)、(124.0±8.2)箇,在AR對照組分彆為(43.2±7.6)、(34.5±5.0)箇,差異有統計學意義(t值分彆為24.260、29.525,P值均＜0.05);MMP-9暘性細胞數在AS組鼻黏膜和肺組織中分彆為(149.9±11.7)、(120.1±7.3)箇,在AS對照組分彆為(48.6±7.6)、(39.1±5.2)箇,差異有統計學意義(t值分彆為22.929、28.530,P值均＜0.05).TNF-α暘性細胞數在AR組鼻黏膜和肺組織中分彆為(188.8±17.0)、(134.8±7.9)箇,在AR對照組分彆為(57.6±23.3)、(40.3±8.2)箇,差異有統計學意義(t值分彆為13.836、26.220,P值均＜0.05);TNF-α暘性細胞數在AS組鼻黏膜和肺組織中分彆為(179.2±15.4)、(153.5±10.1)箇,在AS對照組分彆為(70.5±33.1)、(33.8±14.0)箇,差異有統計學意義(t值分彆為9.412、21.858,P值均＜0.05).MMP-9與TNF-α在AR組鼻黏膜及肺組織中的錶達分彆呈正相關(r值分彆為0.893和0.700,P值分彆為0.001和0.024),二者在AS組鼻黏膜或肺組織中的錶達分彆呈正相關(r值分彆為0.692和0.644,P值分彆為0.027和0.044).結論 上下呼吸道炎性反應具有一緻性,MMP-9和TNF-α可能在上下呼吸道炎性反應一緻性中髮揮重要作用.
목적 통과건립변응성비염(allergic rhinitis,AR)화지기관효천(간칭효천,asthma,AS)동물모형,탐토기질금속단백매9(matrix metalloproteinase-9,MMP-9)화종류배사인자α(tumor necrosis factor,TNF-α)재상하호흡도염성반응일치성중적작용급궤제.방법 이란청단백보이경양화려치민병격발제성AR화AS대서모형.HE염색화갑분알람염색분별검측AR화AS대서모형비점막급폐조직중기산립세포、비대세포적표체,면역조화SP법검측상술조직중MMP-9화TNF-α적표체,분석기산립세포、비대세포、MMP-9화TNF-α표체여상하호흡도염성반응적관계.채용SPSS13.0연건대수거진행통계학분석.결과 MMP-9양성세포수재AR조비점막화폐조직중분별위 (154.8±12.0)、(124.0±8.2)개,재AR대조조분별위(43.2±7.6)、(34.5±5.0)개,차이유통계학의의(t치분별위24.260、29.525,P치균＜0.05);MMP-9양성세포수재AS조비점막화폐조직중분별위(149.9±11.7)、(120.1±7.3)개,재AS대조조분별위(48.6±7.6)、(39.1±5.2)개,차이유통계학의의(t치분별위22.929、28.530,P치균＜0.05).TNF-α양성세포수재AR조비점막화폐조직중분별위(188.8±17.0)、(134.8±7.9)개,재AR대조조분별위(57.6±23.3)、(40.3±8.2)개,차이유통계학의의(t치분별위13.836、26.220,P치균＜0.05);TNF-α양성세포수재AS조비점막화폐조직중분별위(179.2±15.4)、(153.5±10.1)개,재AS대조조분별위(70.5±33.1)、(33.8±14.0)개,차이유통계학의의(t치분별위9.412、21.858,P치균＜0.05).MMP-9여TNF-α재AR조비점막급폐조직중적표체분별정정상관(r치분별위0.893화0.700,P치분별위0.001화0.024),이자재AS조비점막혹폐조직중적표체분별정정상관(r치분별위0.692화0.644,P치분별위0.027화0.044).결론 상하호흡도염성반응구유일치성,MMP-9화TNF-α가능재상하호흡도염성반응일치성중발휘중요작용.
Objective To investigate the role and mechanism of matrix metalloproteinase 9(MMP-9) and tumor necrosis factor α (TNF-α) in the pathogenesis of allergic rhinitis (AR) and asthma (AS). Methods The rat models of AR and AS were made by injecting ovalbumin. The infiltration of eosinophils and mast cells were detected by hematoxylin-eosin (HE) staining and toluidine blue staining respectively, and the expression of MMP-9 and TNF-α in nasal mucosa and lung tissue were examined by immunohistochemical staining ( SP method). The relationship of their expression with upper and lower respiratory tract inflammation was analyzed. SPSS 13.0 software was used to analyze the data. Results The numbers of MMP-9 positive inflammatory cells in nasal mucosa and lung tissue of AR were ( 154.8±12.0)and (124. 0 ±8.2), (43. 2 ±7.6) and (34. 5 ±5.0) in the control groups, the difference was significant (t value were 24. 260, 29. 525 respectively, all P＜0.05). The numbers of MMP-9 positive inflammatory cells in nasal mucosa and lung tissue of AS were (149.9±11.7)and(120.1±7.3), (48.6 ± 7. 6) and (39.1±5.2)in control groups, the difference was significant (t value were 22. 929 and 28. 530respectively, all P＜0.05).The numbers of TNF-α positive inflammatory cells in nasal mucosa and lung tissue of AR were (188.8±17.0), and (134.8±7.9), (57.6±23.3)and(40. 3 ± 8. 2 ) in control groups, the difference was significant (t value were 13. 836 and 26. 220, all P ＜0.05). The numbers of TNF-α positive inflammatory cells in nasal mucosa and lung tissue of AS were (179. 2 ± 15.4 ) and ( 153. 5 ± 10. 1 ), (70. 5 ±33. 1 ) and ( 33.8 ± 14. 0) in control groups, the difference was significant ( t value were 9. 412 and 21. 858, all P ＜0. 05). There was a correlation between the expression of MMP-9 and TNF-α in nasal mucosa and lung tissue of AR ( r values were 0. 893 and 0. 700 respectively, P values were 0. 001 and 0. 024, respectively ) and AS ( r values were 0. 692 and 0. 644 respectively, P values were 0. 027and 0. 044 respectively) groups. Conclusions The inflammation is similar between AR and AS. The MMP9 and TNF-α may play an important role in the pathogenesis of upper and lower respiratory tract inflammation.
