中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2009年
4期
339-343
,共5页
潘浩%朱叶飞%祁贤%张永杰%李亮%邓斐%吴斌%王慎骄%朱凤才%汪华
潘浩%硃葉飛%祁賢%張永傑%李亮%鄧斐%吳斌%王慎驕%硃鳳纔%汪華
반호%주협비%기현%장영걸%리량%산비%오빈%왕신교%주봉재%왕화
手足口病%肠道病毒71型%柯萨奇病毒A组16型%序列分析
手足口病%腸道病毒71型%柯薩奇病毒A組16型%序列分析
수족구병%장도병독71형%가살기병독A조16형%서렬분석
Hand-foot-and-mouth disease%Enterovirus type 71%Coxsackie A16 virus%Sequence analysis
目的 了解2008年江苏省某福利院手足口病(HFMD)暴发疫情的流行病学和病原学特征.方法 对该福利院首起暴发疫情开展流行病学调查,并进行3个月的跟踪随访.实验方法采用荧光定量RT-PCR法检测肠道病毒71型(EV71)和柯萨奇病毒A组16型(CA16)病毒,并用RD、Vero细胞分离病毒,间接免疫荧光法鉴定分离株,并对EV71分离株的VPI基因序列进行种系发生分析.结果 该福利院首起暴发疫情发病数为21例,罹患率为20.0%;10份粪便标本分离出3株EV71病毒,其VPI基因序列分析表明为CA基因亚型,与安徽省阜阳地区08株的同源性在97.9%～100.0%之间.随访研究发现EV71病毒在疫情第1周即可通过粪便排毒,最长排毒期近10周.首起疫情发生2个月后,该福利院又暴发产生6例CA16病毒感染的病例,两起疫情均有3例发生潜伏期排毒,7名婴幼儿被发现机体同时存在两种病毒.结论 该福利院先后两起疫情分别由EV71和CA16病毒引起.EV71分离株为CA基因亚型,同安徽省阜阳地区08株有较高的同源性.两种病毒在机体中存在潜伏期排毒、混合感染(或携带)和超长排毒期现象.
目的 瞭解2008年江囌省某福利院手足口病(HFMD)暴髮疫情的流行病學和病原學特徵.方法 對該福利院首起暴髮疫情開展流行病學調查,併進行3箇月的跟蹤隨訪.實驗方法採用熒光定量RT-PCR法檢測腸道病毒71型(EV71)和柯薩奇病毒A組16型(CA16)病毒,併用RD、Vero細胞分離病毒,間接免疫熒光法鑒定分離株,併對EV71分離株的VPI基因序列進行種繫髮生分析.結果 該福利院首起暴髮疫情髮病數為21例,罹患率為20.0%;10份糞便標本分離齣3株EV71病毒,其VPI基因序列分析錶明為CA基因亞型,與安徽省阜暘地區08株的同源性在97.9%～100.0%之間.隨訪研究髮現EV71病毒在疫情第1週即可通過糞便排毒,最長排毒期近10週.首起疫情髮生2箇月後,該福利院又暴髮產生6例CA16病毒感染的病例,兩起疫情均有3例髮生潛伏期排毒,7名嬰幼兒被髮現機體同時存在兩種病毒.結論 該福利院先後兩起疫情分彆由EV71和CA16病毒引起.EV71分離株為CA基因亞型,同安徽省阜暘地區08株有較高的同源性.兩種病毒在機體中存在潛伏期排毒、混閤感染(或攜帶)和超長排毒期現象.
목적 료해2008년강소성모복리원수족구병(HFMD)폭발역정적류행병학화병원학특정.방법 대해복리원수기폭발역정개전류행병학조사,병진행3개월적근종수방.실험방법채용형광정량RT-PCR법검측장도병독71형(EV71)화가살기병독A조16형(CA16)병독,병용RD、Vero세포분리병독,간접면역형광법감정분리주,병대EV71분리주적VPI기인서렬진행충계발생분석.결과 해복리원수기폭발역정발병수위21례,리환솔위20.0%;10빈분편표본분리출3주EV71병독,기VPI기인서렬분석표명위CA기인아형,여안휘성부양지구08주적동원성재97.9%～100.0%지간.수방연구발현EV71병독재역정제1주즉가통과분편배독,최장배독기근10주.수기역정발생2개월후,해복리원우폭발산생6례CA16병독감염적병례,량기역정균유3례발생잠복기배독,7명영유인피발현궤체동시존재량충병독.결론 해복리원선후량기역정분별유EV71화CA16병독인기.EV71분리주위CA기인아형,동안휘성부양지구08주유교고적동원성.량충병독재궤체중존재잠복기배독、혼합감염(혹휴대)화초장배독기현상.
Objective To determine the epidemiological features of hand-foot-and-mouth disease (HFMD) outbreaks and the genetic characteristics of enterovirus type 71 (EV71) isolates from patients in Lianyungang, Jiangsu province in May, 2008. Methods Epidemiological, microbiological, cellular and molecular methods were performed to investigate pathogens and to describe the homogeneity of isolated strains. Results 21 cases were reported in this HFMD outbreak with the attack rate as 20.0%. 3 EV71 virus strains were isolated from 10 stool samples. The nucleotide and amino acid homogeneity of these 3 Jiangsu strain with Anhui Fuyang strains were 97.9%-100.0% and 99.7%-100.0%, respectively. These 4 Jiangsu strains were within genotype C sub-geno group C4 in phylogenetic tree. Data from the follow-up study showed that shedding of EV71 and Coxsackie A 16 virus (CA 16) in the latent period appeared in the outbreak of HFMD. Human beings could be infected by both EV71 virus and CA16 at the same time and could also carry the two viruses. We also discovered that EV71 virus could be expelled out of the human body through stool in the fast week and last for 10 weeks. Conclusion The recently identified EV71 isolates from this HFMD outbreak belonged to sub-geno group CA. Facts as: the release of viruses in the latent period, co-infection or coexisting of two viruses at the same time and super long period of expulsion of toxin exist in EV71 and CA16 did exist.
