CAJ | 학술논문

目的检测肾综合征出血热(HFRS)患者循环内皮细胞(CEC)中磷酸化黏着斑激酶910(FAKps910)的表达.方法 50例HFRS患者按病情分为轻型17例,中型20例,危重型13例,另纳入健康对照组20例.Percoll密度梯度离心法分离内皮细胞,流式细胞技术检测APC-CD31特异性标记的CEC中FAKps910的表达.数据行单因素方差分析.结果发热早期CEC中FAKps910阳性表达率为j9.87%±9.58%,健康对照组为21.14%±2.53%,发热期末或低血压休克期为11.64%±2.17%(F=262.31,P＜0.01),以后恢复至正常.发热期末或低血压休克期CEC中FAKps910的阳性率在轻型患者为17.45%±2.64%,中型为13.84%±2.54%,危重型为7.47%±2.57%,各型及与健康对照组之间差异均有统计学意义(F=52.642,P＜0.01).结论 HFRS患者CEC中黏着斑激酶(FAK)与病情轻重有关,FAKps910可能参与汉坦病毒引起的83整合素介导的血管内皮细胞损伤.
목적 검측신종합정출혈열(HFRS)환자순배내피세포(CEC)중린산화점착반격매910(FAKps910)적표체.방법 50례HFRS환자안병정분위경형17례,중형20례,위중형13례,령납입건강대조조20례.Percoll밀도제도리심법분리내피세포,류식세포기술검측APC-CD31특이성표기적CEC중FAKps910적표체.수거행단인소방차분석.결과 발열조기CEC중FAKps910양성표체솔위j9.87%±9.58%,건강대조조위21.14%±2.53%,발열기말혹저혈압휴극기위11.64%±2.17%(F=262.31,P＜0.01),이후회복지정상.발열기말혹저혈압휴극기CEC중FAKps910적양성솔재경형환자위17.45%±2.64%,중형위13.84%±2.54%,위중형위7.47%±2.57%,각형급여건강대조조지간차이균유통계학의의(F=52.642,P＜0.01).결론 HFRS환자CEC중점착반격매(FAK)여병정경중유관,FAKps910가능삼여한탄병독인기적83정합소개도적혈관내피세포손상.
Objective To study the expression of phosphorylated serine 910 of focal adhesion kinase(FAKps910)in circulation endothelial cells(CEC)from patients with hemorrhagic fever with renal syndrome(HFRS).Methods Fifty HFRS patients were involved in the study and divided into mild(n=17),moderate(n=20)and severe(n=13)groups according to patients' conditions. Twenty healthy volunteers were enrolled as control group. CEC were isolated by Percoll density gradient centrifugation method. The expression of FAKps910 in specific APC-CD31+ CEC was measured by flow cytometry. The data were analyzed by one-way ANOVA. Results The positive rates of FAKps910 in CEC were 59.87%±9.58% in early febrile stage patients and 21.14%±2.53% in the healthy controls, while it was 11.64%±2.17%in the later febrile or hypotension stage patients(F=262.31,P＜0.01).It gradually restored to normal afterwards. The positive rates of FAKps910 in CEC were 17.45%±2.64%,13.84%±2.54% and 7.47%±2.57%,respectively in mild, morderate and severe groups in the later febrile or hypotension stage, which were all significantly different from that in control group(F=52.642,P＜0.01).Conclusions FAK in CEC iS related with the clinical severity of HFRS patients. FAK may be involved in intergrin β3-mediated endothelial injury during Hantavirus infection.