CAJ | 학술논문

目的研究活性氧簇(ROS)对3T3-LI脂肪细胞脂联素表达的调节机制.方法以实时定量PCR检测分化成熟的3T3-L1脂肪细胞脂联素mRNA表达水平,联合应用多重磷酸化蛋白分析系统与各种蛋白激酶抑制剂,筛查ROS下调脂联索表达的信号通路.结果作为一种重要的ROS,H_2O_2激活了313-L1脂肪细胞细胞外信号调节激酶1/2(ERK1/2)、c-Jun氨基端激酶(JNK)、蛋白激酶B(Akt)、p70 S6 激酶(p70 S6K)及Janus激酶/信号转导和转录激活因子(JAK/STAT)等多种信号转导通路.其中Akt和JAK/STAT抑制剂完全逆转H_2_O2对脂联素表达的下调作用(均P<0.01).结论 ROS可能通过激活Akt、JAK/STAT信号途径下调脂肪细胞脂联素的表达,从而在肥胖及其相关疾病的发生、发展中发挥作用.
목적 연구활성양족(ROS)대3T3-LI지방세포지련소표체적조절궤제.방법 이실시정량PCR검측분화성숙적3T3-L1지방세포지련소mRNA표체수평,연합응용다중린산화단백분석계통여각충단백격매억제제,사사ROS하조지련색표체적신호통로.결과 작위일충중요적ROS,H_2O_2격활료313-L1지방세포세포외신호조절격매1/2(ERK1/2)、c-Jun안기단격매(JNK)、단백격매B(Akt)、p70 S6 격매(p70 S6K)급Janus격매/신호전도화전록격활인자(JAK/STAT)등다충신호전도통로.기중Akt화JAK/STAT억제제완전역전H_2_O2대지련소표체적하조작용(균P<0.01).결론 ROS가능통과격활Akt、JAK/STAT신호도경하조지방세포지련소적표체,종이재비반급기상관질병적발생、발전중발휘작용.
Objective To investigate the signaling cascades involved in the regulatory effects of reactive oxygen species(ROS)on adiponectin expression in 3T3-L1 adipocytes.Methods 3T3-L1 cells were cultured and differentiated into mature adipocytes.Adiponectin mRNA expression was evaluated by quantitative real-time PCR.The signaling pathways associated with ROS-decreased adiponectin expression were screened by Bioplex phosphoprotein assays and various protein kinase inhibitors.Results As an important ROS,H_2O_2activated several signaling pathways including extracellular signal regulated protein kinase 1/2(ERK1/2),c-Jun N-terminal kinase (JNK),protein kinase B(Akt),p70 S6 kinase(p70 S6K),and Janus kinase/signal transducer and activator of transcription(JAK/STAT).Akt and JAK/STAT inhibitors completely reversed H_2O_2-decreased adiponectin expression(both P<0.01).Conclusions ROS markedly down-regulates adiponeetin expression in 313-L1 adipocytes by activating Akt and JAK/STAT signaling pathways,which may contribute to the development of obesity and its related diseases.