CAJ | 학술논문

目的探讨脑肿瘤干细胞(BTSCs)移植瘤组织中的肿瘤细胞、血管内皮细胞的起源问题,旨在阐明BTSCs在脑肿瘤组织重构中所起的作用. 方法 BTSCs球体接种到NC系裸小鼠右尾状核,在出现恶病质时处死,取移植瘤组织常规石蜡包埋、切片后进行HE染色和人类白细胞抗原(HLA)染色,在光学显微镜下观察. 结果 HE染色见肿瘤细胞广泛播散在宿主的脑组织中.HLA染色显示存在肿瘤细胞直接构成的血管. 结论在BTSCs的裸小鼠移植瘤中.肿瘤细胞在宿主脑内广泛分布并构成了血管;BTSCs可能转分化成肿瘤血管细胞,在脑胶质瘤组织重构中起重要作用.
목적 탐토뇌종류간세포(BTSCs)이식류조직중적종류세포、혈관내피세포적기원문제,지재천명BTSCs재뇌종류조직중구중소기적작용. 방법 BTSCs구체접충도NC계라소서우미상핵,재출현악병질시처사,취이식류조직상규석사포매、절편후진행HE염색화인류백세포항원(HLA)염색,재광학현미경하관찰. 결과 HE염색견종류세포엄범파산재숙주적뇌조직중.HLA염색현시존재종류세포직접구성적혈관. 결론 재BTSCs적라소서이식류중.종류세포재숙주뇌내엄범분포병구성료혈관;BTSCs가능전분화성종류혈관세포,재뇌효질류조직중구중기중요작용.
Objective To investigate the origin of tumor cells and vascular endothelial cells in intraeranial brain tumor stem cell (BTSC) xenografls and elucidate the role of BTSCs in brain tumor tissue remodeling. Methods BTSC spheres were injected into the right candate nucleus of nude mice, and the mice were sacrificed after the occurrence ofcachexia to obtain the tumor tissue. Routine paraffin embedding, slicing, HE staining and human leucocyte antigen (HLA) staining of the tumor tissues were performed for observation of the tumor tissues under optical microscope. Results HE staining displayed dissemination growth of the tumor cells in the host brain tissue. HLA staining revealed the presence of blood vessels constituted directly by the tumor cells. Conclusion In the intracranial xenografts of BTSCs, the tumor cells are extensively distributed and constitute the blood vessels. The BTSCs play an important role in glioma tissue remodeling by differentiating into vascular endothelial cells in the tumors.