CAJ | 학술논문

目的探讨系统性红斑狼疮(SLE)患者抗磷脂综合征(APS)的继发与血清肿瘤坏死因子α(TNFα)、P-选择素(P-selectin)水平的关系.方法采用改良Russell蝰蛇毒稀释试验(DRVVT)测定41例SLE患者及25例正常人血浆狼疮抗凝物质(LAC),酶联免疫吸附试验(ELISA)方法检测血清抗心磷脂-IgG、IgM(ACL-IgG、IgM)抗体,ELISA方法测定SLE患者及正常对照组血清P-selectin、TNFα.结果 (1)41例SLE患者中19例血浆中检测到LAC和(或)血清中检测到高滴度ACL-IgG、IgM;(2)与正常对照组(10.58±4.10) ng/L相比,SLE继发APS组、未继发APS组的P-selectin水平均升高,分别为(21.55±6.29) ng/L和(14.02±9.48) ng/L,SLE未继发APS组与继发APS组相比,P-selectin水平差异亦有统计学意义;(3)仅SLE继发APS组(30.01±13.3) pg/ml与正常对照组间(21.60±12.78) pg/ml TNFα的差异有统计学意义,其余各组间差异均无统计学意义;(4)各组内血清P-selectin水平与TNFα水平均无相关性.结论在SLE患者继发APS的病程中,P-selectin起到重要作用,且P-selectin的作用强于TNFα.
목적 탐토계통성홍반랑창(SLE)환자항린지종합정(APS)적계발여혈청종류배사인자α(TNFα)、P-선택소(P-selectin)수평적관계.방법 채용개량Russell호사독희석시험(DRVVT)측정41례SLE환자급25례정상인혈장랑창항응물질(LAC),매련면역흡부시험(ELISA)방법검측혈청항심린지-IgG、IgM(ACL-IgG、IgM)항체,ELISA방법측정SLE환자급정상대조조혈청P-selectin、TNFα.결과 (1)41례SLE환자중19례혈장중검측도LAC화(혹)혈청중검측도고적도ACL-IgG、IgM;(2)여정상대조조(10.58±4.10) ng/L상비,SLE계발APS조、미계발APS조적P-selectin수평균승고,분별위(21.55±6.29) ng/L화(14.02±9.48) ng/L,SLE미계발APS조여계발APS조상비,P-selectin수평차이역유통계학의의;(3)부SLE계발APS조(30.01±13.3) pg/ml여정상대조조간(21.60±12.78) pg/ml TNFα적차이유통계학의의,기여각조간차이균무통계학의의;(4)각조내혈청P-selectin수평여TNFα수평균무상관성.결론 재SLE환자계발APS적병정중,P-selectin기도중요작용,차P-selectin적작용강우TNFα.
Objective To investigate the relationship between anti-phospholipid syndrome(APS) in systemic lupus erythematosus(SLE) and serum levels of P-selectin and TNFα.Methods Lupus anti-coagulant(LAC) were detected using plasma from 41 SLE patients and 25 healthy controls by simplified dilute russell′s viper venom test(DRVVT).Serum levels of anticardiolipin-IgG,IgM(ACL-IgG,IgM) antibody,P-selectin and TNFα were studied by enzyme-linked immunosorbent assay(ELISA).Results (1)LAC and(or) high titers ACL-IgG,IgM antibodies were detected in 19 patients of 41 SLE patients.(2)The levels of P-selectin was significantly higher in SLE with APS group[(21.55±6.29) ng/L]and SLE free of APS group (14.02±9.48) ng/L than that in normal group(10.58±4.10) ng/L respectively.The difference of serum P-selectin concentrations between SLE with APS group and SLE free of APS group was also significant.(3)Comparing serum TNFα concentrations between different groups,only that in SLE with APS group(30.01±13.3)pg/ml was significantly higher than that in normal group(21.60±12.78)pg/ml.(4)There was no correlation between P-selectin levels and TNFα levels within each group. Conclusion P-selectin plays more important role than TNFα in SLE patients complication with APS.