CAJ | 학술논문

目的:建立一种快速而准确的检测血清成骨生长肽浓度的方法.方法:制备具有较高效价的成骨生长肽抗体,通过竞争性酶联免疫吸附法及其改进方法建立检测成骨生长肽浓度的标准曲线.结果:亲和纯化的抗体比盐析抗体的标准曲线更好,改进的竞争性酶联免疫吸附法方法比普通的竞争性酶联免疫吸附法方法的检测灵敏度高一个数量级,可满足临床检测血清成骨生长肽浓度灵敏度的要求.结论:改进的竞争性酶联免疫吸附法可以为临床研究提供可靠而灵敏地检测血清成骨生长肽含量的方法.
목적:건립일충쾌속이준학적검측혈청성골생장태농도적방법.방법:제비구유교고효개적성골생장태항체,통과경쟁성매련면역흡부법급기개진방법건립검측성골생장태농도적표준곡선.결과:친화순화적항체비염석항체적표준곡선경호,개진적경쟁성매련면역흡부법방법비보통적경쟁성매련면역흡부법방법적검측령민도고일개수량급,가만족림상검측혈청성골생장태농도령민도적요구.결론:개진적경쟁성매련면역흡부법가이위림상연구제공가고이령민지검측혈청성골생장태함량적방법.
Objective:To develop an enzyme immunoassay for the detection of Osteogenic Growth Peptide in serum for clinical investigation.Methods:Two kinds of competitive Enzyme linked immununosorbent assay were employed,based on sensitivity and shape of dose-reaction curve,the most suitable testing pattern was selected for further application.Results:Purified antibody was better than salting-out antibody in competitive Enzyme linked immununosorbent assay in terms of standard curve.Modified competitive Enzyme linked immununosorbent assay was better than competitive Enzyme linked immununosorbent assay in sensitivity.Conclusion:Modified competitive Enzyme linked immununosorbent assay for Osteogenic Growth Peptide is sensitive enough for clinical investigation.