CAJ | 학술논문

目的观测表没食子儿茶素没食子酸酯(epigallocatechin-3-gallate,EGCG)对人大肠癌SW620细胞增殖及SW620细胞表达PAK1的影响.方法用终浓度为40 μmol/L、60 μmol/L和80 ixmo1/L的EGCG干预人大肠癌细胞株SW620,在0、24、48和72 h不同时间点上用噻唑蓝(MTT)比色法检测EGCG对SW620细胞生长的影响;并以SW620细胞空白组为对照,用蛋白免疫印迹(Westerm blot)检测不同EGCG作用48 h时SW620细胞PAK1的表达水平变化.结果 EGCG对大肠癌SW620细胞增殖有显著的抑制作用,而且与SW620细胞空白对照组相比.EGCG处理组细胞的PAK1蛋白表达受到显著抑制.结论 EGCG抑制大肠癌SW620细胞增殖的机制可能与EGCG通过调控大肠癌细胞的PAK1表达相关.
목적 관측표몰식자인다소몰식자산지(epigallocatechin-3-gallate,EGCG)대인대장암SW620세포증식급SW620세포표체PAK1적영향.방법 용종농도위40 μmol/L、60 μmol/L화80 ixmo1/L적EGCG간예인대장암세포주SW620,재0、24、48화72 h불동시간점상용새서람(MTT)비색법검측EGCG대SW620세포생장적영향;병이SW620세포공백조위대조,용단백면역인적(Westerm blot)검측불동EGCG작용48 h시SW620세포PAK1적표체수평변화.결과 EGCG대대장암SW620세포증식유현저적억제작용,이차여SW620세포공백대조조상비.EGCG처리조세포적PAK1단백표체수도현저억제.결론 EGCG억제대장암SW620세포증식적궤제가능여EGCG통과조공대장암세포적PAK1표체상관.
Objective To investigate the effects of epigallocatechin-3-gallate (EGCG) on the proliferation of SW620 cells and the expression of PAK1 gene. Methods Human colonic cancer cell line SW620 was treated with EGCG at 40, 60 and 80 μmol/L and cultured in RPMI 1640 medium for 0, 24, 48 and 72 h. The proliferation of SW620 cells was observed by MTT assay before and after EGCG treatment, and the expression of PAK1 protein was observed by Western blotting. Results SW620 cells treated with EGCG displayed a slowed growth in comparison with the control cells, and the growth rate decreased with the increase of EGCG concentration. PAK1 protein expression was lowered in SW62Q, cells after EGCG treatment for 48 h. Conclusion EGCG can inhibit the proliferation and partially reduce the expression of PAK1 protein in SW620 cells.