CAJ | 학술논문

[目的]克隆与分析亚洲黑熊四川亚种线粒体NADH的脱氢酶亚基5和亚基6基因(ND5和ND6).[方法]根据已报道的部分熊科动物ND5和ND6基因序列的相关信息设计引物,运用PCR技术,首次从亚洲黑熊四川亚种的肌肉组织总DNA中成功克隆了ND5和ND6基因的编码序列,分析了其序列特征,并与已报道的9种熊科动物进行比较分析.[结果]该克隆片段包括ND5和ND6两个基因共2 456 bp,共编码781个氨基酸,与9种熊科动物的ND5和ND6具有很高的同源性;该克隆片段ND5和ND6的蛋白分子量分别为68.262 1和19.038 6 kDa,等电点分别为9.19和4.25,与9种熊科动物的均十分接近.[结论]四川黑熊与9种熊科动物的ND5和ND6基因及其编码蛋白具有很高的相似性,在功能上具有一致性,尤其与东北黑熊在功能上具有高度一致性.
[목적]극륭여분석아주흑웅사천아충선립체NADH적탈경매아기5화아기6기인(ND5화ND6).[방법]근거이보도적부분웅과동물ND5화ND6기인서렬적상관신식설계인물,운용PCR기술,수차종아주흑웅사천아충적기육조직총DNA중성공극륭료ND5화ND6기인적편마서렬,분석료기서렬특정,병여이보도적9충웅과동물진행비교분석.[결과]해극륭편단포괄ND5화ND6량개기인공2 456 bp,공편마781개안기산,여9충웅과동물적ND5화ND6구유흔고적동원성;해극륭편단ND5화ND6적단백분자량분별위68.262 1화19.038 6 kDa,등전점분별위9.19화4.25,여9충웅과동물적균십분접근.[결론]사천흑웅여9충웅과동물적ND5화ND6기인급기편마단백구유흔고적상사성,재공능상구유일치성,우기여동북흑웅재공능상구유고도일치성.
[Objective] The purpose was to clone and analyze mitochondrial NADH dehydrogenase subunits 5 and 6 (ND5 and ND6) genes of Ursus thibetanus spp. mupinensis. [Method] The primers were designed according to the reported information related to ND5 and ND6 gene sequence of part Ursid. The coding sequences of ND5 and ND6 genes were cloned in the total DNA in musculature of mupinensis successfully for the first time by applying PCR technique and their sequence characteristics were analyzed and compared with that of 9 reported Ursid species. [Result] This cloned sequence included 2 genes of ND5 and ND6, their total length was 2 456 bp and they coded 781 amino acids and had very high homology with ND5 and ND6 genes of the 9 Ursid species. The molecular weights of proteins coded by ND5 and ND6 genes of this cloned sequence were 68.262 1 and 19.038 6 kDa resp. and their isoelectric points were 9.19 and 4.25 resp. Their molecular weights and isoelectric points were very close to that of the 9 Ursid species. [Conclusion] The ND5 and ND6 genes and their coded proteins of mupinensis had very high similarity and functional consistency with that of the 9 Ursid species, especially high functional consistency with that of Selenarctos thibetanus G. Cuvier.