CAJ | 학술논문

目的探讨广西巴马长寿地区老年人肾虚证候与血管紧张素转换酶(ACE)基因插入/缺失(I/D)多态性的关系.方法采用问卷调查并采血进行聚合酶链反应(PCR)、单链构象多态性分析(SSCP)与直接测序技术检验确认,研究百岁组(≥100岁)27名、90岁组(90～99岁)56名、老年组(60～89岁)114名老人的肾虚证候及其与ACE基因I/D多态性的关系,以79例60～70岁自然死亡人群为对照.结果 D/D型老年人肾虚症状最少,I/D型老年人次之,I/I型老年人肾虚症状最多.在ACE基因I/D多态基因型中,百岁组I/I、I/D、D/D型频率分别为37,04%(10/27)、33.33%(9/27)和29.63%(8/27),I和D等位基因频率分别为53.70%(29/54)和46.30%(25/54).百岁组D/D基因型频率和D型等位基因频率高于老年组及对照组(P＜0.05,P＜0.01),但与90岁组比较,无论是I/I、I/D或D/D基因型,所占百分率均比较接近,差异无统计学意义(P＞0.05).D/D基因型经SSCP证实全部相同,无差别.直接测序结果再次验证ACE(I/D)多态性判断准确.结论老年肾虚证候与ACE基因I/D多态性及长寿密切相关,同时也应考虑到长寿受多种因素影响.
목적 탐토엄서파마장수지구노년인신허증후여혈관긴장소전환매(ACE)기인삽입/결실(I/D)다태성적관계.방법 채용문권조사병채혈진행취합매련반응(PCR)、단련구상다태성분석(SSCP)여직접측서기술검험학인,연구백세조(≥100세)27명、90세조(90～99세)56명、노년조(60～89세)114명노인적신허증후급기여ACE기인I/D다태성적관계,이79례60～70세자연사망인군위대조.결과 D/D형노년인신허증상최소,I/D형노년인차지,I/I형노년인신허증상최다.재ACE기인I/D다태기인형중,백세조I/I、I/D、D/D형빈솔분별위37,04%(10/27)、33.33%(9/27)화29.63%(8/27),I화D등위기인빈솔분별위53.70%(29/54)화46.30%(25/54).백세조D/D기인형빈솔화D형등위기인빈솔고우노년조급대조조(P＜0.05,P＜0.01),단여90세조비교,무론시I/I、I/D혹D/D기인형,소점백분솔균비교접근,차이무통계학의의(P＞0.05).D/D기인형경SSCP증실전부상동,무차별.직접측서결과재차험증ACE(I/D)다태성판단준학.결론 노년신허증후여ACE기인I/D다태성급장수밀절상관,동시야응고필도장수수다충인소영향.
Objective To explore the relationship between Shen-deficiency syndrome (SDS) and the insert/deficit (I/D) polymorphism types (PMTs) of angiotensin converting enzyme (ACE) gene in longevous elders in Bama area of Guangxi municipality.Methods The investigation on 27 centenarians(Group A),56 elders of 90-99 years old (Group B) and 114 of 60-89 years old (Group C) was carried out by questionnaire,and the polymorphism was detected using polymerize chain reaction detection,single strand conformation polymorphism (SSCP) analysis,and direct sequencing technique.And the study was controlled by 79 naturally died elders within 60-70 years old.Results The presenting frequencies of SDS in various PMT were D/D,I/D and I/I in rising order.The frequencies of PMT,I/I,I/D and D/D,were 37.04%(10/27),33.33% (9/27),and 29.63%(8/27) respectively;the frequency rates of I and D alleles were 53.70% (29/54) and 46.30% (25/54) in Group A.Comparison of frequency of D/D and D allele between groups showed that Group A was significantly different to Group C (P＜0.05) and also to the control (P＜0.01),while all the frequencies in Group A and B were rather identical,showing insignificant difference (P＞0.05).SSCP analysis showed no significant difference in D/D among groups.Outcomes of direct sequencing test further proved the correctness of I/D ACE polymorphism detection.Conclusion SDS is closely correlated with ACE gene polymorphism and life span.Whereas,other multiple factors that influence longevity should also be taken into consideration.